P2Y1/P2RY1 Antibody (HL2702)
Novus Biologicals | Catalog # NBP3-48720
Recombinant Monoclonal Antibody
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Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL2702
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Product Specifications
Immunogen
Synthetic peptide encompassing a sequence within the Intracellular domain of human P2Y1/P2RY1. The exact sequence is proprietary.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for P2Y1/P2RY1 Antibody (HL2702)
Immunohistochemistry-Paraffin: P2Y1/P2RY1 Antibody (HL2702) [NBP3-48720] -
P2Y1/P2RY1 antibody [HL2702] detects P2Y1/P2RY1 protein by immunohistochemical analysis.Sample: Paraffin-embedded human pancreas.
P2Y1/P2RY1 stained by P2Y1/P2RY1 antibody [HL2702] (NBP3-48720) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: P2Y1/P2RY1 Antibody (HL2702) [NBP3-48720] -
Boiled and unboiled LoVo whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y1/P2RY1 antibody [HL2702] (NBP3-48720) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.Western Blot: P2Y1/P2RY1 Antibody (HL2702) [NBP3-48720] -
Non-transfected (-) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y1/P2RY1 antibody [HL2702] (NBP3-48720) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Western Blot: P2Y1/P2RY1 Antibody (HL2702) [NBP3-48720] -
Unboiled Non-transfected (-) and transfected (+) 293T and Expi293 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y1/P2RY1 antibody [HL2702] (NBP3-48720) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for P2Y1/P2RY1 Antibody (HL2702)
Application
Recommended Usage
Western Blot
1:5000-1:50000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: P2Y1/P2RY1
Long Name
P2Y Purinergic Receptor 1
Alternate Names
P2RY1
Gene Symbol
P2RY1
Additional P2Y1/P2RY1 Products
Product Documents for P2Y1/P2RY1 Antibody (HL2702)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for P2Y1/P2RY1 Antibody (HL2702)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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