p40/deltaNp63 Antibody (P40/7302R)
Novus Biologicals | Catalog # NBP3-13806
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # P40/7302R
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Product Specifications
Immunogen
Recombinant fragment (around aa1-200) of human p40/deltaNp63 protein (exact sequence is proprietary) (Uniprot: Q9H3D4 )
Localization
Nucleus
Marker
Squamous, Basal & Myoepithelial Cell Marker
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Theoretical MW
63 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified by Protein A Column. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14057)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for p40/deltaNp63 Antibody (P40/7302R)
![Immunohistochemistry-Paraffin: p40/deltaNp63 Antibody (P40/7302R) [NBP3-13806]](https://resources.rndsystems.com/images/products/p40-deltaNp63-Antibody-P40-7302R-Immunohistochemistry-Paraffin-NBP3-13806-img0002.jpg "Immunohistochemistry-Paraffin: p40/deltaNp63 Antibody (P40/7302R) [NBP3-13806]")
Immunohistochemistry-Paraffin: p40/deltaNp63 Antibody (P40/7302R) [NBP3-13806]
Immunohistochemistry-Paraffin: p40/deltaNp63 Antibody (P40/7302R) [NBP3-13806] - Formalin-fixed, paraffin-embedded human prostate cancer stained with p40/deltaNp63 antibody (P40/7302R). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.![Immunohistochemistry-Paraffin: p40/deltaNp63 Antibody (P40/7302R) [NBP3-13806]](https://resources.rndsystems.com/images/products/p40-deltaNp63-Antibody-P40-7302R-Immunohistochemistry-Paraffin-NBP3-13806-img0001.jpg "Immunohistochemistry-Paraffin: p40/deltaNp63 Antibody (P40/7302R) [NBP3-13806]")
Immunohistochemistry-Paraffin: p40/deltaNp63 Antibody (P40/7302R) [NBP3-13806]
Immunohistochemistry-Paraffin: p40/deltaNp63 Antibody (P40/7302R) [NBP3-13806] - Formalin-fixed, paraffin-embedded human prostate cancer stained with p40/deltaNp63 antibody (P40/7302R). Inset: PBS instead of primary antibody, secondary antibody negative control.![Immunohistochemistry-Paraffin: p40/deltaNp63 Antibody (P40/7302R) [NBP3-13806]](https://resources.rndsystems.com/images/products/p40-deltaNp63-Antibody-P40-7302R-Immunohistochemistry-Paraffin-NBP3-13806-img0003.jpg "Immunohistochemistry-Paraffin: p40/deltaNp63 Antibody (P40/7302R) [NBP3-13806]")
Immunohistochemistry-Paraffin: p40/deltaNp63 Antibody (P40/7302R) [NBP3-13806]
Immunohistochemistry-Paraffin: p40/deltaNp63 Antibody (P40/7302R) [NBP3-13806] - Formalin-fixed, paraffin-embedded human ovarian cancer stained with p40/deltaNp63 antibody (P40/7302R). Inset: PBS instead of primary antibody; secondary only negative control.Applications for p40/deltaNp63 Antibody (P40/7302R)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: p40/deltaNp63
Alternate Names
AIS, amplified in squamous cell carcinoma, B(p51A), B(p51B), Chronic ulcerative stomatitis protein, EEC3, Keratinocyte transcription factor KET, KET, LMS, OFC8, p53CP, SHFM4, TP53CP, TP53L, TP63, TP73L, Transformation-related protein 63, tumor protein p53-competing protein
Gene Symbol
TP63
UniProt
Additional p40/deltaNp63 Products
Product Documents for p40/deltaNp63 Antibody (P40/7302R)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for p40/deltaNp63 Antibody (P40/7302R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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