p400 Antibody

Novus Biologicals | Catalog # NB200-210

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Bovine (100%), Chimpanzee (100%), Equine (100%), Guinea Pig (100%), Monkey (100%), Porcine (100%), Primate (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all p400 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 3075 and the C-terminus (residue 3124) of human E1A binding protein p400 using the numbering given in entry AAK97789.1 (GeneID 57634).

Reactivity Notes

Gorilla (100%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for p400 Antibody

Western Blot: p400 Antibody [NB200-210]

Western Blot: p400 Antibody [NB200-210]

Western Blot: p400 Antibody [NB200-210] - Detection of mouse p400 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-p400 antibody NB200-210 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: p400 Antibody [NB200-210]

Western Blot: p400 Antibody [NB200-210]

Western Blot: p400 Antibody [NB200-210] - Detection of human p400 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, K-562, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-p400 antibody NB200-210 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: p400 Antibody [NB200-210]

Immunoprecipitation: p400 Antibody [NB200-210]

Immunoprecipitation: p400 Antibody [NB200-210] - Detection of human p400 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-p400 antibody NB200-210 (lot 2) used for IP at 6 ug per reaction. p400 was also immunoprecipitated by a previous lot of this antibody (lot 1) and rabbit anti-p400 antibody from Company B. For blotting immunoprecipitated p400, NB200-210 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for p400 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:5000
Application Notes
Centrifuge tube to remove product from lid. Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: p400

P400 protein is a 250 kd glycoprotein, characteristic of the cerebellum, which is accumulated at the endoplasmic reticulum, at the plasma membrane and at the post-synaptic density of Purkinje cells.

Alternate Names

CAG repeat protein 32, CAGH32hDomino, DKFZP434I225, Domino homolog, E1A binding protein p400, E1A-binding protein p400, EC 3.6.1, EC 3.6.4.-, FLJ45115, KIAA1498p400 kDa SWI2/SNF2-related protein, KIAA1818, P400, p400 SWI2/SNF2-related protein, TNRC12FLJ42018, trinucleotide repeat containing 12, Trinucleotide repeat-containing gene 12 protein

Entrez Gene IDs

57634 (Human)

Gene Symbol

EP400

UniProt

AAK97789.1 (Human)

Additional p400 Products

Product Documents for p400 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for p400 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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