p400 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-88230

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all p400 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DDEVDDEEETIEEEEANEGVVDHQTELSNLAKEAELPLLDLMKLYEGAFLPSSQWPRPKPDGEDTSGEEDADDCPGDRESRKDLVLIDSLFIMDQFKAAERMNIGKPNAKDIADVTAVAEAILPKGSARVTTSVKFNAPSL

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (82%), Rat (84%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for p400 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: p400 Antibody [NBP1-88230]

Immunocytochemistry/ Immunofluorescence: p400 Antibody [NBP1-88230]

Immunocytochemistry/Immunofluorescence: p400 Antibody [NBP1-88230] - Staining of human cell line U-2 OS shows localization to nucleus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: p400 Antibody [NBP1-88230]

Immunohistochemistry-Paraffin: p400 Antibody [NBP1-88230]

Immunohistochemistry-Paraffin: p400 Antibody [NBP1-88230] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
p400 Antibody - BSA Free Immunohistochemistry-Paraffin: p400 Antibody - BSA Free [NBP1-88230]

Immunohistochemistry-Paraffin: p400 Antibody - BSA Free [NBP1-88230]

Staining of human cerebral cortex shows moderate nuclear/nucleoli positivity in neurons.
p400 Antibody - BSA Free Immunohistochemistry-Paraffin: p400 Antibody - BSA Free [NBP1-88230]

Immunohistochemistry-Paraffin: p400 Antibody - BSA Free [NBP1-88230]

Staining of human rectum shows moderate nuclear/nucleoli positivity in glandular cells.
p400 Antibody - BSA Free Immunohistochemistry-Paraffin: p400 Antibody - BSA Free [NBP1-88230]

Immunohistochemistry-Paraffin: p400 Antibody - BSA Free [NBP1-88230]

Staining of human skin shows moderate nuclear/nucleoli positivity in squamous epithelial cells.

Applications for p400 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: p400

P400 protein is a 250 kd glycoprotein, characteristic of the cerebellum, which is accumulated at the endoplasmic reticulum, at the plasma membrane and at the post-synaptic density of Purkinje cells.

Alternate Names

CAG repeat protein 32, CAGH32hDomino, DKFZP434I225, Domino homolog, E1A binding protein p400, E1A-binding protein p400, EC 3.6.1, EC 3.6.4.-, FLJ45115, KIAA1498p400 kDa SWI2/SNF2-related protein, KIAA1818, P400, p400 SWI2/SNF2-related protein, TNRC12FLJ42018, trinucleotide repeat containing 12, Trinucleotide repeat-containing gene 12 protein

Gene Symbol

EP400

Additional p400 Products

Product Documents for p400 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for p400 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for p400 Antibody - BSA Free

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Protocols

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