Human p53 ELISA Kit (Colorimetric)

Key Product Details

Product Specifications

Precision

Intra-Assay Precision (Precision within an assay) CV% 5.50%

Inter-Assay Precision (Precision between assays) CV% 8.90%

Recovery for Human p53 ELISA Kit (Colorimetric)

Scientific Data Images for Human p53 ELISA Kit (Colorimetric)

Kit Contents for Human p53 ELISA Kit (Colorimetric)

• Adhesive Films
• Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human p53
• Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
• Biotin-Conjugate anti-human p53 monoclonal antibody
• Human p53 Standard lyophilized, 100 U/ml upon reconstitution
• Sample Diluent
• Stop Solution (1M Phosphoric acid)
• Streptavidin-HRP
• Substrate Solution (tetramethyl-benzidine)
• Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Background: p53

p53 is a tumor suppressor that is regulated by transcriptional, post-transcriptional, and post-translational mechanisms in response to stress. Studies demonstrate that p53 is critical for the prevention of tumorigenesis and may potentially be involved in at least half of all known cancers. p53 functions as a transcription factor to regulate genes involved in apoptosis, cell cycle, senescence, metabolism, angiogenesis, immunity, differentiation, and migration. Moreover, p53 is phosphorylated at multiple sites, including Ser15, following DNA damage.

Additional p53 Products

• All Products for p53
• p53 Activity Assays
• p53 cDNA Clones
• p53 ELISA Kits
• p53 Lysates
• p53 Primary Antibodies
• p53 Proteins and Enzymes

Product Documents for Human p53 ELISA Kit (Colorimetric)