Rat p53 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-75359

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

46.88 pg/mL

Assay Range

78.13 - 5000 pg/mL
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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Rat

Specificity

This kit recognizes Rat TP53 in samples. No significant cross-reactivity or interference between Rat TP53 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 4.54%

Inter-Assay Precision (Precision between assays) CV% 4.94%

Recovery for Rat p53 ELISA Kit (Colorimetric)

Recovery

88-105%

Linearity

Rat p53 ELISA Kit (Colorimetric)

Scientific Data Images for Rat p53 ELISA Kit (Colorimetric)

Rat p53 ELISA Kit (Colorimetric)

ELISA: Rat p53 ELISA Kit (Colorimetric) [NBP2-75359] -

ELISA: Rat p53 ELISA Kit (Colorimetric) [NBP2-75359] - Standard Curve Reference

Kit Contents for Rat p53 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: p53

p53 is a stress-regulated transcription factor that was first identified as an SV40 large T antigen-binding protein. It plays a major role in the cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair, or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro. Mutation of p53 is the most common genetic change so far identified in a number of major carcinomas. This antibody can be used for the specific detection of human p53 that is synthesized in the presence of p53 from other species.

Alternate Names

BCC7, LFS1, TP53, TRP53

Gene Symbol

TP53

Additional p53 Products

Product Documents for Rat p53 ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Rat p53 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Citations for Rat p53 ELISA Kit (Colorimetric)

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