p63/TP73L Antibody (TP63/2428)
Novus Biologicals | Catalog # NBP3-07443
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin, Flow Cytometry, Protein Array
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # TP63/2428
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Product Specifications
Immunogen
Recombinant full-length human p63/TP73L protein (Uniprot: Q9H3D4 )
Localization
Nuclear
Marker
Squamous, Basal & Myoepithelial Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Theoretical MW
63 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08736)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for p63/TP73L Antibody (TP63/2428)
![Immunohistochemistry-Paraffin: p63/TP73L Antibody (TP63/2428) [NBP3-07443]](https://resources.rndsystems.com/images/products/p63-TP73L-Antibody-TP63-2428-Immunohistochemistry-Paraffin-NBP3-07443-img0003.jpg "Immunohistochemistry-Paraffin: p63/TP73L Antibody (TP63/2428) [NBP3-07443]")
Immunohistochemistry-Paraffin: p63/TP73L Antibody (TP63/2428) [NBP3-07443]
Immunohistochemistry-Paraffin: p63/TP73L Antibody (TP63/2428) [NBP3-07443] - Formalin-fixed, paraffin-embedded human prostate carcinoma stained with p63/TP73L Mouse Monoclonal Antibody (TP63/2428).![Flow Cytometry: p63/TP73L Antibody (TP63/2428) [NBP3-07443]](https://resources.rndsystems.com/images/products/p63-TP73L-Antibody-TP63-2428-Flow-Cytometry-NBP3-07443-img0002.jpg "Flow Cytometry: p63/TP73L Antibody (TP63/2428) [NBP3-07443]")
Flow Cytometry: p63/TP73L Antibody (TP63/2428) [NBP3-07443]
Flow Cytometry: p63/TP73L Antibody (TP63/2428) [NBP3-07443] - Flow Cytometric Analysis of PFA-fixed HeLa cells using p63/TP73L Mouse Monoclonal Antibody (TP63/2428) followed by goat anti- Mouse IgG-CF488 (blue); isotype control (red).![Protein Array: p63/TP73L Antibody (TP63/2428) [NBP3-07443]](https://resources.rndsystems.com/images/products/p63-TP73L-Antibody-TP63-2428-Protein-Array-NBP3-07443-img0005.jpg "Protein Array: p63/TP73L Antibody (TP63/2428) [NBP3-07443]")
Protein Array: p63/TP73L Antibody (TP63/2428) [NBP3-07443]
Protein Array: p63/TP73L Antibody (TP63/2428) [NBP3-07443] - Analysis of Protein Array containing more than 19,000 full-length human proteins using p63/TP73L Antibody (TP63/2428).
Immunohistochemistry-Paraffin: p63/TP73L Antibody (TP63/2428) [NBP3-07443] -
Immunohistochemistry-Paraffin: p63/TP73L Antibody (TP63/2428) [NBP3-07443] - Formalin-fixed, paraffin-embedded human basal cell carcinoma stained with p63/TP73L Mouse Monoclonal Antibody (TP63/2428).Applications for p63/TP73L Antibody (TP63/2428)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: p63/TP73L
Long Name
Tumor Protein 63
Alternate Names
EEC3, KET, OFC8, p40, p51, p73L, SHFM4, TP63, TP73L
Gene Symbol
TP63
UniProt
Additional p63/TP73L Products
Product Documents for p63/TP73L Antibody (TP63/2428)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for p63/TP73L Antibody (TP63/2428)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
