p67phox/NOXA2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-94271

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 227-526 of human p67phox/NOXA2 (NP_000424.2). PPPRPKTPEIFRALEGEAHRVLFGFVPETKEELQVMPGNIVFVLKKGNDNWATVMFNGQKGLVPCNYLEPVELRIHPQQQPQEESSPQSDIPAPPSSKAPGRPQLSPGQKQKEEPKEVKLSVPMPYTLKVHYKYTVVMKTQPGLPYSQVRDMVSKKLELRLEHTKLSYRPRDSNELVPLSEDSMKDAWGQVKNYCLTLWCENTVGDQGFPDEPKESEKADANNQTTEPQLKKGSQVEALFSYEATQPEDLEFQEGDIILVLSKVNEEWLEGECKGKVGIFPKVFVEDCATTDLESTRREV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit p67phox/NOXA2 Antibody - BSA Free (NBP2-94271) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for p67phox/NOXA2 Antibody - BSA Free

Western Blot: p67phox/NOXA2 AntibodyBSA Free [NBP2-94271]

Western Blot: p67phox/NOXA2 AntibodyBSA Free [NBP2-94271]

Western Blot: p67phox/NOXA2 Antibody [NBP2-94271] - Analysis of extracts of various cell lines, using p67phox/NOXA2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry-Paraffin: p67phox/NOXA2 Antibody - BSA Free [NBP2-94271]

Immunohistochemistry-Paraffin: p67phox/NOXA2 Antibody - BSA Free [NBP2-94271]

Immunohistochemistry-Paraffin: p67phox/NOXA2 Antibody [NBP2-94271] - Immunohistochemistry of paraffin-embedded human esophageal cancer using p67phox/NOXA2 Rabbit pAb (NBP2-94271) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
p67phox/NOXA2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: p67phox/NOXA2 Antibody - BSA Free [p67phox/NOXA2] -

Immunocytochemistry/ Immunofluorescence: p67phox/NOXA2 Antibody - BSA Free [p67phox/NOXA2] - Immunofluorescence analysis of Human spleen tissue using p67phox/NOXA2 Rabbit pAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining.

Applications for p67phox/NOXA2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: p67phox

NOXA2 encodes neutrophil cytosolic factor 2, the 67-kilodalton cytosolic subunit of the multi-protein NADPH oxidase complex found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in this gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms. Alternative splicing results in two transcript variants that encode the same protein.

Alternate Names

NCF2, NOXA2

Gene Symbol

NCF2

Additional p67phox Products

Product Documents for p67phox/NOXA2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for p67phox/NOXA2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Neutrophils

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Protocols

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