PABPC4 Antibody

Western Blot: PABPC4 Antibody [NB100-74594]

Western Blot: PABPC4 Antibody [NB100-74594] - Detection of Human and Mouse PABP4 on HeLa whole cell lysate using NB100-74594. PABP4 was also immunoprecipitated by rabbit anti-PABP4 antibody NB100-74593.

Immunohistochemistry-Paraffin: PABPC4 Antibody [NB100-74594]

Immunohistochemistry-Paraffin: PABPC4 Antibody [NB100-74594] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- PABP4 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Western Blot: PABPC4 Antibody [NB100-74594] -

Depletion of PABPC in mammalian cell lines has minimal effect on TE.(A) The effect of PABPC knockdown on protein synthesis in HeLa cells. Cells transfected with the indicated siRNAs were cultured for 48 hr, then treated with puromycin before harvesting for western-blot analysis. In a control sample, cycloheximide (CHX) was added prior to puromycin treatment. At the top is the membrane showing total protein levels. In the middle is the same membrane probed for puromycin to detect nascent protein synthesis. At the bottom are the results of the same membrane probed for the indicated proteins. (B) The effect of PABPC knockdown on polysomes in HeLa cells. At the top are polysome-gradient profiles from HeLa cells transfected with either control siRNAs or siRNAs targeting PABPC1 and PABPC4. At the bottom are northern blots analyzing RNA collected from gradient fractions shown at the top, probing for four cytoplasmic mRNAs and one mitochondrial mRNA (MT-CYB). (C) The early effects of rapid PABPC1 degradation on mRNA abundance (left), ribosome-footprint abundance (middle) and TE (right) in HCT116 PABPC1-AID cells. At the top, values from cells treated with IAA for 0.5 hr are compared to those from cells not treated with IAA. In the middle, values from cells treated with IAA for 1 hr are compared to those from cells not treated with IAA. At the bottom, values from cells treated with IAA for 3 hr are compared to those from cells not treated with IAA. For each gene, values for mRNA and ribosome-footprint reads per kilobase are plotted after normalizing to values for mitochondrial mRNAs. These results are summarized Figure 6F. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34213414), licensed under a CC-BY license. Not internally tested by Novus Biologicals.