PAF1 Antibody - BSA Free
Novus Biologicals | Catalog # NB600-274
![Immunohistochemistry-Paraffin: PAF1 Antibody [NB600-274]](https://resources.rndsystems.com/images/products/PAF1-Antibody-Immunohistochemistry-Paraffin-NB600-274-img0010.jpg "Immunohistochemistry-Paraffin: PAF1 Antibody [NB600-274]")
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)
Cited:
Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
A synthetic peptide which maps to a region between residues 475 and 525 of human RNA Polymerase Associated Factor using the numbering given in TrEMBL entry Q9NUU9 (GeneID 54623).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PAF1 Antibody - BSA Free
Immunohistochemistry-Paraffin: PAF1 Antibody [NB600-274]
Immunohistochemistry-Paraffin: PAF1 Antibody [NB600-274] - Section of mouse teratoma. Antibody: Affinity purified rabbit anti- Paf1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB![Immunohistochemistry-Paraffin: PAF1 Antibody [NB600-274]](https://resources.rndsystems.com/images/products/PAF1-Antibody-Immunohistochemistry-Paraffin-NB600-274-img0009.jpg "Immunohistochemistry-Paraffin: PAF1 Antibody [NB600-274]")
Immunohistochemistry-Paraffin: PAF1 Antibody [NB600-274]
Immunohistochemistry-Paraffin: PAF1 Antibody [NB600-274] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti- Paf1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB![Immunoprecipitation: PAF1 Antibody [NB600-274]](https://resources.rndsystems.com/images/products/PAF1-Antibody-Immunoprecipitation-NB600-274-img0008.jpg "Immunoprecipitation: PAF1 Antibody [NB600-274]")
Immunoprecipitation: PAF1 Antibody [NB600-274]
Immunoprecipitation: PAF1 Antibody [NB600-274] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Paf1 antibody NB600-274 Lot 2 used for IP at 3 ug/mg lysate. Paf1 was also immunoprecipitated by rabbit anti-Paf1 Lot 1 of the same product. For blotting immunoprecipitated Paf1, NB600-274 Lot 2 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time 30 seconds.![Immunoprecipitation: PAF1 Antibody [NB600-274]](https://resources.rndsystems.com/images/products/PAF1-Antibody-Immunoprecipitation-NB600-274-img0011.jpg "Immunoprecipitation: PAF1 Antibody [NB600-274]")
Immunoprecipitation: PAF1 Antibody [NB600-274]
Immunoprecipitation: PAF1 Antibody [NB600-274] - Detection of human Paf1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Paf1 antibody NB600-274 (lot NB600-274-3) used for IP at 6 ug per reaction. Paf1 was also immunoprecipitated by a previous lot of this antibody (lot NB600-274-2) and rabbit anti-Paf1 antibody NB600-273. For blotting immunoprecipitated Paf1, NB600-274 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Applications for PAF1 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:500-1:2000
Immunohistochemistry-Paraffin
1:10-1:500
Immunoprecipitation
3-10 ug/mg lysate
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. For better Western Blot antibody see NB 600-273 instead. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: PAF1
Alternate Names
F23149_1, FLJ11123, Paf1, RNA polymerase II associated factor, homolog (S. cerevisiae), Pancreatic differentiation protein 2, PD2hPAF1, RNA polymerase II-associated factor 1 homolog
Entrez Gene IDs
54623 (Human)
Gene Symbol
PAF1
UniProt
Additional PAF1 Products
Product Documents for PAF1 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for PAF1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for PAF1 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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