PAG1 Antibody (MEM-255) - BSA Free

Novus Biologicals | Catalog # NB500-342

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Bovine (Negative), Mouse (Negative), Rat (Negative)

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow (Intracellular), CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # MEM-255

Format

BSA Free
View all PAG1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant intracellular fragment, corresponding to amino acids 97-432 of Human Cbp/PAG (NM_018440.3).

Specificity

The antibody MEM-255 recognizes an epitope (aa 235-280) of Csk-binding protein (Cbp) located in the cytoplasmic domain, also known as protein associated with glycosphingolipid-enriched microdomains (PAG).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for PAG1 Antibody (MEM-255) - BSA Free

Immunohistochemistry: PAG1 Antibody (MEM-255) - BSA Free [NB500-342]

Immunohistochemistry: PAG1 Antibody (MEM-255) - BSA Free [NB500-342]

Immunohistochemistry: PAG Antibody (MEM-255) [NB500-342] - Cbp/PAG is expressed in germinal centers of lymph node lymphoid follicle and in follicular lymphoma (it is absent from mantle zone). Cbp/PAG is also expressed more weakly in T cells in tonsil and the thymic medulla.

Applications for PAG1 Antibody (MEM-255) - BSA Free

Application
Recommended Usage

Flow (Intracellular)

2 ug/ml

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:200

Western Blot

1:1000
Application Notes
Purity is > 95% (by SDS-PAGE). Flow Cytometry - intracellular staining, permeabilization required Western Blot - Csk binding protein is an ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane microdomains (rafts), which however migrates on SDS-PAGE gels anomalously as an 80 kDa molecule. This antibody is CyTOF ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PAG1

PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.

Long Name

Phosphoprotein Associated with Glycosphingolipid Microdomains 1

Alternate Names

CBP

Entrez Gene IDs

55824 (Human); 94212 (Mouse); 64019 (Rat)

Gene Symbol

PAG1

UniProt

Q9NWQ8 (Human)

Additional PAG1 Products

Product Documents for PAG1 Antibody (MEM-255) - BSA Free

Certificate of Analysis

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Product Specific Notices for PAG1 Antibody (MEM-255) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Mast Cells

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Protocols

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