PAG1 Antibody - BSA Free

Novus Biologicals | Catalog # NB500-541

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PAG1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant intracellular fragment (aa 97-432) of human Cbp (PAG).

Specificity

The polyclonal antibody recognizes Csk-binding protein (Cbp / PAG), a 46 kDa ubiquitously expressed transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit PAG1 Antibody - BSA Free (NB500-541) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for PAG1 Antibody - BSA Free

PAG1 Antibody Western Blot: PAG1 Antibody [NB500-541]

Western Blot: PAG1 Antibody [NB500-541]

Analysis of human PAG using rabbit polyclonal antibody PAb (409) on lysates of Daudi and Ramos cell line under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of rabbit anti-PAG monoclonal antibody followed by IRDye800-conjugated anti-rabbit secondary antibody. PAG was detected around 65 kDa.

Applications for PAG1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:200

Immunoprecipitation

1:50

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry-Paraffin: Positive tissue: tonsil, colon germinal center.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PAG1

PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.

Long Name

Phosphoprotein Associated with Glycosphingolipid Microdomains 1

Alternate Names

CBP

Gene Symbol

PAG1

Additional PAG1 Products

Product Documents for PAG1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PAG1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Mast Cells

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Protocols

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