PAK1 Antibody (6R3M5)

Name: PAK1 Antibody (6R3M5)
Brand: Novus Biologicals
SKU: NBP3-15811
Price: 389 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-15811

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6R3M5 expressed in HEK293

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Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PAK1 (Q13153). MSNNGLDIQDKPPAPPMRNTSTMIGAGSKDAGTLNHGSKPLPPNPEEKKKKDRFYRSILPGDKTNKKKEKERPEISLPSDFEHTIHVGFDAVTGEFTGMP

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PAK1 Antibody (6R3M5)

Western Blot: PAK1 Antibody (6R3M5) [NBP3-15811]

Western Blot: PAK1 Antibody (6R3M5) [NBP3-15811] - Western blot analysis of extracts of various cell lines, using PAK1 antibody (NBP3-15811) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunocytochemistry/ Immunofluorescence: PAK1 Antibody (6R3M5) [NBP3-15811]

Immunocytochemistry/Immunofluorescence: PAK1 Antibody (6R3M5) [NBP3-15811] - Immunofluorescence analysis of NIH-3T3 cells using PAK1 antibody (NBP3-15811). Blue: DAPI for nuclear staining.

Applications for PAK1 Antibody (6R3M5)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PAK1

p21-activated kinase 1 (PAK1) is a member of a family of serine/threonine protein kinase defined by their interaction with the small GTPases, Cdc42 and Rac (1-2). The PAK proteins can be essentially divided into two categories, groups I (PAK1, PAK2 and PAK3) and group II (PAK4, PAK5 and PAK6), based on their structures (3). PAK1 participates in regulating the actin cytoskeleton, focal adhesion contacts, cell motility, apoptosis and transcription. It also promotes the disassembly of stress fibers (4-5). PAK1 also binds PIX (PAK-binding protein), COOL and adapter protein Nck (5).

Long Name

p21/Cdc42/Rac1-activated Kinase 1

Alternate Names

alpha-PAK

Gene Symbol

PAK1

Additional PAK1 Products

Product Documents for PAK1 Antibody (6R3M5)

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Certificate of Analysis

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Product Specific Notices for PAK1 Antibody (6R3M5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Intracellular Kinases

PDGF Family

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