PAK1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-04201

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PAK1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human PAK1 (Q13153). VYTRSVIEPLPVTPTRDVATSPISPTENNTTPPDALTRNTEKQKKKPKMSDEEILEKLRSIVSVGDPKKKYTRFEKIGQGASGTVYTAMDVATGQEVAIKQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PAK1 Antibody - BSA Free

Western Blot: PAK1 AntibodyBSA Free [NBP3-04201]

Western Blot: PAK1 AntibodyBSA Free [NBP3-04201]

Western Blot: PAK1 Antibody [NBP3-04201] - Analysis of extracts of mouse kidney, using PAK1 antibody at 1:700 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry-Paraffin: PAK1 Antibody - BSA Free [NBP3-04201]

Immunohistochemistry-Paraffin: PAK1 Antibody - BSA Free [NBP3-04201]

Immunohistochemistry-Paraffin: PAK1 Antibody [NBP3-04201] - Paraffin-embedded human lung cancer using PAK1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry-Paraffin: PAK1 Antibody - BSA Free [NBP3-04201]

Immunohistochemistry-Paraffin: PAK1 Antibody - BSA Free [NBP3-04201]

Immunohistochemistry-Paraffin: PAK1 Antibody [NBP3-04201] - Paraffin-embedded rat spleen using PAK1 antibody at dilution of 1:100 (40x lens).

Applications for PAK1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:25-1:100

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PAK1

p21-activated kinase 1 (PAK1) is a member of a family of serine/threonine protein kinase defined by their interaction with the small GTPases, Cdc42 and Rac (1-2). The PAK proteins can be essentially divided into two categories, groups I (PAK1, PAK2 and PAK3) and group II (PAK4, PAK5 and PAK6), based on their structures (3). PAK1 participates in regulating the actin cytoskeleton, focal adhesion contacts, cell motility, apoptosis and transcription. It also promotes the disassembly of stress fibers (4-5). PAK1 also binds PIX (PAK-binding protein), COOL and adapter protein Nck (5).

Long Name

p21/Cdc42/Rac1-activated Kinase 1

Alternate Names

alpha-PAK

Gene Symbol

PAK1

Additional PAK1 Products

Product Documents for PAK1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PAK1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Intracellular Kinases
PDGF Family

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Protocols

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FAQs

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