PAK2 Antibody (2Y7I2)

Novus Biologicals | Catalog # NBP3-16433

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2Y7I2 expressed in HEK293
View all PAK2 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PAK2 (Q13177). MSDNGELEDKPPAPPVRMSSTIFSTGGKDPLSANHSLKPLPSVPEEKKPRHKIISIFSGTEKGSKKKEKERPEISPPSDFEHTIHVGFDAVTGEFTGMPE

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PAK2 Antibody (2Y7I2)

Western Blot: PAK2 Antibody (2Y7I2) [NBP3-16433]

Western Blot: PAK2 Antibody (2Y7I2) [NBP3-16433]

Western Blot: PAK2 Antibody (2Y7I2) [NBP3-16433] - Western blot analysis of extracts of various cell lines, using PAK2 Rabbit mAb (NBP3-16433) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunocytochemistry/ Immunofluorescence: PAK2 Antibody (2Y7I2) [NBP3-16433]

Immunocytochemistry/ Immunofluorescence: PAK2 Antibody (2Y7I2) [NBP3-16433]

Immunocytochemistry/Immunofluorescence: PAK2 Antibody (2Y7I2) [NBP3-16433] - Immunofluorescence analysis of U2OS cells using PAK2 antibody (NBP3-16433) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: PAK2 Antibody (2Y7I2) [NBP3-16433]

Immunocytochemistry/ Immunofluorescence: PAK2 Antibody (2Y7I2) [NBP3-16433]

Immunocytochemistry/Immunofluorescence: PAK2 Antibody (2Y7I2) [NBP3-16433] - Immunofluorescence analysis of C6 cells using PAK2 antibody (NBP3-16433) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: PAK2 Antibody (2Y7I2) [NBP3-16433]

Immunocytochemistry/ Immunofluorescence: PAK2 Antibody (2Y7I2) [NBP3-16433]

Immunocytochemistry/Immunofluorescence: PAK2 Antibody (2Y7I2) [NBP3-16433] - Immunofluorescence analysis of NIH/3T3 cells using PAK2 antibody (NBP3-16433) at dilution of 1:100. Blue: DAPI for nuclear staining.

Applications for PAK2 Antibody (2Y7I2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PAK2

p21-activated kinase 2 (PAK2) is a member of a family of serine/threonine protein kinase defined by their interaction with the small GTPases, Cdc42 and Rac (1-2). The PAK proteins can be essentially divided into two categories, groups I (PAK1, PAK2 and PAK3) and group II (PAK4, PAK5 and PAK6), based on their structures (3). All six PAKs play an important role in diverse cellular processes, including cytoskeletal dynamics, growth/apoptotic signal transduction through MAP kinases, and regulation of transcription factors (4). The ubiquitous PAK-2 is unique among the PAK isoforms because it is also activated through proteolytic cleavage by caspases or caspase-like proteases (5). Full-length PAK-2 is localized in the cytoplasm, whereas the proteolytic PAK-2p34 fragment translocates to the nucleus. Activation of full-length PAK-2 stimulates cell survival, whereas proteolytic activation of PAK-2p34 is involved in programmed cell death (6).

Long Name

p21-activated Kinase 2

Alternate Names

gamma-PAK, PAK65, S6/H4 Kinase

Gene Symbol

PAK2

Additional PAK2 Products

Product Documents for PAK2 Antibody (2Y7I2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for PAK2 Antibody (2Y7I2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics
Intracellular Kinases

Customer Reviews for PAK2 Antibody (2Y7I2)

There are currently no reviews for this product. Be the first to review PAK2 Antibody (2Y7I2) and earn rewards!

Have you used PAK2 Antibody (2Y7I2)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies