pan Nuclear Pore Complex Marker Antibody (39C7)
Novus Biologicals | Catalog # NBP1-05390
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Key Product Details
Species Reactivity
Human, Mouse, Rat, Porcine, Bovine, C. elegans, Chicken, Drosophila, Equine, Yeast
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 39C7
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Product Specifications
Immunogen
Yeast nuclear preparations
Specificity
Panspecific
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for pan Nuclear Pore Complex Marker Antibody (39C7)
Western Blot: pan Nuclear Pore Complex Marker Antibody (39C7) [NBP1-05390]
Western Blot: pan Nuclear Pore Complex Marker Antibody (39C7) [NBP1-05390] - Analysis of different cell lysates, cytosol or nuclear enriched fractions using pan-specific mouse mAb to the nuclear pore complex (NPC), NBP1-05390, dilution 1:100 in green: [1] protein standard (red), [2] HEK293 cytosol, [3] HEK293 nuclear, [4] NIH-3T3 cytosol, [5] NIH-3T3 nuclear, [6] HeLa cytosol, and [7] HeLa nuclear fraction lysate. The band at about 68kDa represents a currently unidentified NPC protein which is detected predominantly in the nuclear enriched fractions of all cell lines.Immunocytochemistry/ Immunofluorescence: pan Nuclear Pore Complex Marker Antibody (39C7) [NBP1-05390]
Immunocytochemistry/Immunofluorescence: pan Nuclear Pore Complex Marker Antibody (39C7) [NBP1-05390] - HeLa cells were stained with monoclonal antibody NBP1-05390, which binds to a nuclear pore complex antigen, and chicken antibody to vimentin NBP1-05425.
pan Nuclear Pore Complex Marker Antibody (39C7) [NBP1-05390] - Analysis of HeLa cells stained with panspecificmouse mAb to the nuclear pore complex (NPC), NBP1-05390, dilution 1:100 in red, and costained with chicken pAb to vimentin, dilution 1:10,000, in green. The blue is DAPI staining of nuclear DNA. The NBP1-05390 antibody reveals strong granular staining of the nuclei corresponding to the NPC, while the vimentin antibody specifically labels intermediate filaments in these cells.
Applications for pan Nuclear Pore Complex Marker Antibody (39C7)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:500
Western Blot
1:100
Application Notes
This pan Nuclear Pore Complex Marker (39C7) antibody is useful for Western Blot and Immunocytochemistry/Immunofluorescence. For ICC/IF the recommended dilutions are 1:100 to 1:500 (yeast cells) and 1:50 to 1:100 (mammalian cells).
Formulation, Preparation, and Storage
Purification
Tissue culture supernatant
Formulation
Supplied as concentrated hybridoma cell culture media.
Preservative
0.035% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: pan Nuclear Pore Complex Marker
Alternate Names
107 kDa nucleoporin, NPC proteins, Nuclear pore complex protein Nup107, Nucleoporin 107kDa, Nucleoporin Nup107, NUP 107, NUP 84, NUP107, NUP84
Additional pan Nuclear Pore Complex Marker Products
Product Documents for pan Nuclear Pore Complex Marker Antibody (39C7)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for pan Nuclear Pore Complex Marker Antibody (39C7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for pan Nuclear Pore Complex Marker Antibody (39C7)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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