PAPD1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-35779

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PAPD1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 383-582 of human PAPD1 (NP_060579.3).

Sequence:
MVIFFLQRRSPPILPTLDSLKTLADAEDKCVIEGNNCTFVRDLSRIKPSQNTETLELLLKEFFEYFGNFAFDKNSINIRQGREQNKPDSSPLYIQNPFETSLNISKNVSQSQLQKFVDLARESAWILQQEDTDRPSISSNRPWGLVSLLLPSAPNRKSFTKKKSNKFAIETVKNLLESLKGNRTENFTKTSGKRTISTQT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PAPD1 Antibody - BSA Free

PAPD1 Antibody

Western Blot: PAPD1 Antibody [NBP3-35779] -

Western Blot: PAPD1 Antibody [NBP3-35779] - Western blot analysis of various lysates using PAPD1 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

Applications for PAPD1 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PAPD1

Polymerase that creates the 3' poly(A) tail of mitochondrial transcripts. Plays a role in replication-dependent histone mRNA degradation. May be involved in the terminal uridylation of mature histone mRNAs before their degradation is initiated. Might be responsible for the creation of some UAA stop codons which are not encoded in mtDNA

Alternate Names

EC 2.7.7.19, FLJ10486, mitochondrial poly(A) polymerase, mtPAP, PAP, PAP associated domain containing 1, PAP-associated domain-containing protein 1, PAPD1SPAX4, poly(A) RNA polymerase, mitochondrial, Polynucleotide adenylyltransferase, Terminal uridylyltransferase 1, TUTase 1

Gene Symbol

MTPAP

Additional PAPD1 Products

Product Documents for PAPD1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PAPD1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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