PAPD4 Antibody

Western Blot: PAPD4 Antibody [NBP3-18647]

Western Blot: PAPD4 Antibody [NBP3-18647] - Figure 2. Western blot analysis using the antibody directed against PAPD4 Nuclear extracts of HeLa cells (40 ug) were analysed by Western blot using the antibody against PAPD4 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 56 kDa); the marker (in kDa) is shown on the left.

ELISA: PAPD4 Antibody [NBP3-18647]

ELISA: PAPD4 Antibody [NBP3-18647] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against human PAPD4. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:107,000.