PAPSS1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-13730

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (98%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PAPSS1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: QERDIVPVDASYEVKELYVPENKLHLAKTDAETLPALKINKVDMQWVQVLAE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PAPSS1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: PAPSS1 Antibody [NBP2-13730]

Immunocytochemistry/ Immunofluorescence: PAPSS1 Antibody [NBP2-13730]

Immunocytochemistry/Immunofluorescence: PAPSS1 Antibody [NBP2-13730] - Staining of human cell line U-2 OS shows localization to nucleus & nucleoli.
Immunohistochemistry-Paraffin: PAPSS1 Antibody [NBP2-13730]

Immunohistochemistry-Paraffin: PAPSS1 Antibody [NBP2-13730]

Immunohistochemistry-Paraffin: PAPSS1 Antibody [NBP2-13730] - Staining of human smooth muscle shows moderate cytoplasmic positivity in smooth muscle cells.
Simple Western: PAPSS1 Antibody [NBP2-13730]

Simple Western: PAPSS1 Antibody [NBP2-13730]

Simple Western: PAPSS1 Antibody [NBP2-13730] - Lane view shows a specific band for PAPSS1 using 200 ug/mL of HepG2 cell lysate and 1:25 antibody dilution. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 65 kDa. Image from an internal validation.

Applications for PAPSS1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200-1:500

Simple Western

1:25
Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

See Simple Western Antibody Database for Simple Western validation: Tested in HepG2 lysate, separated by Size, antibody dilution of 1:25, apparent MW was 65 kDa

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PAPSS1

Three-prime-phosphoadenosine 5-prime-phosphosulfate (PAPS) is the sulfate donor cosubstrate for all sulfotransferase (SULT) enzymes (Xu et al., 2000 [PubMed 10679223]). SULTs catalyze the sulfate conjugation of many endogenous and exogenous compounds, including drugs and other xenobiotics. In humans, PAPS is synthesized from adenosine 5-prime triphosphate (ATP) and inorganic sulfate by 2 isoforms, PAPSS1 and PAPSS2 (MIM 603005).[supplied by OMIM]

Alternate Names

3'-phosphoadenosine 5'-phosphosulfate synthase 1, ATPSK1SK 1, bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1, EC 2.7.1.25, PAPS synthase 1, PAPSS 1, PAPSS3-prime-phosphoadenosine 5-prime-phosphosulfate synthase 1, SK1, Sulfurylase kinase 1

Gene Symbol

PAPSS1

Additional PAPSS1 Products

Product Documents for PAPSS1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PAPSS1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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