PAPSS2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-55135

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PAPSS2 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptides corresponding to PAPSS2(3'-phosphoadenosine 5'-phosphosulfate synthase 2) The peptide sequence was selected from the C terminal of PAPSS2. Peptide sequence PARHNEFDFISGTRMRKLAREGENPPDGFMAPKAWKVLTDYYRSLEKN. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for PAPSS2 Antibody - BSA Free

Western Blot: PAPSS2 Antibody [NBP1-55135]

Western Blot: PAPSS2 Antibody [NBP1-55135]

Western Blot: PAPSS2 Antibody [NBP1-55135] - Human 721_B, Antibody Dilution: 1.0 ug/ml There is BioGPS gene expression data showing that PAPSS2 is expressed in 721_B.
Immunohistochemistry: PAPSS2 Antibody [NBP1-55135]

Immunohistochemistry: PAPSS2 Antibody [NBP1-55135]

Immunohistochemistry: PAPSS2 Antibody [NBP1-55135] - Human Liver Tissue Observed Staining: Cytoplasm in hepatocytes Primary Antibody Concentration: 1 : 100 Other Working Concentrations: 1/600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1 : 200 Magnification: 20X Exposure Tim
Western Blot: PAPSS2 Antibody [NBP1-55135]

Western Blot: PAPSS2 Antibody [NBP1-55135]

Western Blot: PAPSS2 Antibody [NBP1-55135] - 293T cells lysate, concentration 0.2-1 ug/ml.
Western Blot: PAPSS2 Antibody [NBP1-55135]

Western Blot: PAPSS2 Antibody [NBP1-55135]

Western Blot: PAPSS2 Antibody [NBP1-55135] - Human Hela, Antibody Dilution: 1.0 ug/ml PAPSS2 is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells.
Immunohistochemistry: PAPSS2 Antibody [NBP1-55135]

Immunohistochemistry: PAPSS2 Antibody [NBP1-55135]

Immunohistochemistry: PAPSS2 Antibody [NBP1-55135] - Human Lung Tissue Observed Staining: Cytoplasm of pneumocytes Primary Antibody Concentration: 1 : 100 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1 : 200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec.

Applications for PAPSS2 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PAPSS2

Sulfation is a common modification of endogenous (lipids, proteins, and carbohydrates) and exogenous (xenobiotics and drugs) compounds. In mammals, the sulfate source is 3'-phosphoadenosine 5'-phosphosulfate (PAPS), created from ATP and inorganic sulfate. Two different tissue isoforms encoded by different genes synthesize PAPS. PAPSS2 is one of the two PAPS synthetases. Defects in this gene cause the Pakistani type of spondyloepimetaphyseal dysplasia. Two alternatively spliced transcript variants that encode different isoforms have been described for this gene.Sulfation is a common modification of endogenous (lipids, proteins, and carbohydrates) and exogenous (xenobiotics and drugs) compounds. In mammals, the sulfate source is 3'-phosphoadenosine 5'-phosphosulfate (PAPS), created from ATP and inorganic sulfate. Two different tissue isoforms encoded by different genes synthesize PAPS. This gene encodes one of the two PAPS synthetases. Defects in this gene cause the Pakistani type of spondyloepimetaphyseal dysplasia. Two alternatively spliced transcript variants that encode different isoforms have been described for this gene.

Alternate Names

3'-phosphoadenosine 5'-phosphosulfate synthase 2, ATP sulfurylase/adenosine 5'-phosphosulfate kinase, ATPSK2ATP sulfurylase/APS kinase 2, bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2, bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthethase 2, EC 2.7.1.25, PAPS synthase 2, PAPS synthetase 2, PAPSS 2, SK 2, SK2, Sulfurylase kinase 2,3-prime-phosphoadenosine 5-prime-phosphosulfate synthase 2

Gene Symbol

PAPSS2

UniProt

O95340-2 (Human)

Additional PAPSS2 Products

Product Documents for PAPSS2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PAPSS2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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