PAR1/Thrombin Receptor Antibody

Novus Biologicals | Catalog # NBP1-51936

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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

Peptide with sequence C-TRARRPESKATNAT corresponding to internal region (near N-Terminus) according to NP_001983.2.

Epitope

C-TRARRPESKATNAT

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PAR1/Thrombin Receptor Antibody

Western Blot: PAR1/Thrombin Receptor Antibody [NBP1-51936]

Western Blot: PAR1/Thrombin Receptor Antibody [NBP1-51936]

Western Blot: PAR1/Thrombin Receptor Antibody [NBP1-51936] - Staining of HeLa lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence. ~48 kDa band observed (calculated MW of 47.4 kDa band according to NP_001983.2).

Applications for PAR1/Thrombin Receptor Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:64000

Western Blot

0.3 - 1 ug/mL
Application Notes
WB Approx 48kDa band observed in lysates of cell line HeLa (calculated MW of 47.4kDa according to NP_001983.2). Primary incubation 1 hour at room temperature.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PAR1

Thrombin is a serine protease that is involved in platelet aggregation and blood coagulation. It is cleaved from its precursor, prothrombin, and converts fibrinogen to fibrin in the final step of the clotting cascade. Thrombin mediates its regulatory effects by activating cell surface receptors. These receptors, including thrombin receptor (also designated PAR-1, for protease-activated receptor-1), PAR-2 and PAR-3 are members of the G-protein coupled receptor family, and share a similiar gene structure. Thrombin cleaves its receptor, releasing a 41 amino acid peptide which acts as a platelet agonist. Upon this activation by thrombin, the thrombin receptors trigger an increase in cytosolic Ca2+ concentration. Unactivated thrombin receptor cycles between the cell surface and an intracellular pool, while activated receptor internalizes rapidly and is degraded in the lysosomes. The human thrombin receptor is also known to be regulated by Sp1 and Sp3 transcription factors.

Long Name

Protease-Activated Receptor 1

Alternate Names

Cf2r, F2R, HTR, ThrR, TRGPC

Entrez Gene IDs

2149 (Human)

Gene Symbol

F2R

UniProt

NP_001983.2 (Human)

Additional PAR1 Products

Product Documents for PAR1/Thrombin Receptor Antibody

Certificate of Analysis

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Product Specific Notices for PAR1/Thrombin Receptor Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PAR1/Thrombin Receptor Antibody

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