PARG Antibody (OTI6F4)

Novus Biologicals | Catalog # NBP2-46320

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI6F4
View all PARG Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-367 of human PARG(NP_003622) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

110.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PARG Antibody (OTI6F4)

Western Blot: PARG Antibody (OTI6F4) [NBP2-46320]

Western Blot: PARG Antibody (OTI6F4) [NBP2-46320]

Western Blot: PARG Antibody (OTI6F4) [NBP2-46320] - Analysis of extracts (10ug) from 8 different cell lines by using anti-PARG monoclonal antibody.(1:200
Immunohistochemistry-Paraffin: PARG Antibody (OTI6F4) [NBP2-46320]

Immunohistochemistry-Paraffin: PARG Antibody (OTI6F4) [NBP2-46320]

Immunohistochemistry-Paraffin: PARG Antibody (OTI6F4) [NBP2-46320] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Western Blot: PARG Antibody (OTI6F4) [NBP2-46320]

Western Blot: PARG Antibody (OTI6F4) [NBP2-46320]

Western Blot: PARG Antibody (OTI6F4) [NBP2-46320] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PARG.
Immunohistochemistry-Paraffin: PARG Antibody (OTI6F4) [NBP2-46320]

Immunohistochemistry-Paraffin: PARG Antibody (OTI6F4) [NBP2-46320]

Immunohistochemistry-Paraffin: PARG Antibody (OTI6F4) [NBP2-46320] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry-Paraffin: PARG Antibody (OTI6F4) [NBP2-46320]

Immunohistochemistry-Paraffin: PARG Antibody (OTI6F4) [NBP2-46320]

Immunohistochemistry-Paraffin: PARG Antibody (OTI6F4) [NBP2-46320] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry-Paraffin: PARG Antibody (OTI6F4) [NBP2-46320]

Immunohistochemistry-Paraffin: PARG Antibody (OTI6F4) [NBP2-46320]

Immunohistochemistry-Paraffin: PARG Antibody (OTI6F4) [NBP2-46320] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry-Paraffin: PARG Antibody (OTI6F4) [NBP2-46320]

Immunohistochemistry-Paraffin: PARG Antibody (OTI6F4) [NBP2-46320]

Immunohistochemistry-Paraffin: PARG Antibody (OTI6F4) [NBP2-46320] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry-Paraffin: PARG Antibody (OTI6F4) [NBP2-46320]

Immunohistochemistry-Paraffin: PARG Antibody (OTI6F4) [NBP2-46320]

Immunohistochemistry-Paraffin: PARG Antibody (OTI6F4) [NBP2-46320] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry-Paraffin: PARG Antibody (OTI6F4) [NBP2-46320]

Immunohistochemistry-Paraffin: PARG Antibody (OTI6F4) [NBP2-46320]

Immunohistochemistry-Paraffin: PARG Antibody (OTI6F4) [NBP2-46320] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
PARG Antibody (OTI6F4)

PARG Antibody (OTI6F4)[NBP2-46320]

PARG Antibody (OTI6F4)[NBP2-46320]Simple Western� analysis of endogenous protein PHGDH from HEK293T lysates (0.2 mg/mL) using PHGDH Mouse Monoclonal Antibody. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess� Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12�230 kDa Separation Module.

Applications for PARG Antibody (OTI6F4)

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PARG

Poly(ADP-ribose) glycohydrolase (PARG) is the major enzyme responsible for the catabolism of poly(ADP-ribose), a reversible covalent-modifier of chromosomal proteins. The protein is found in many tissues and may be subject to proteolysis generating smaller, active products. [provided by RefSeq]

Long Name

Poly [ADP-ribose] Glycohydrolase

Alternate Names

PARG99

Gene Symbol

PARG

Additional PARG Products

Product Documents for PARG Antibody (OTI6F4)

Certificate of Analysis

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Product Specific Notices for PARG Antibody (OTI6F4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PARG Antibody (OTI6F4)

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Protocols

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