Q: I am doing research on Neuroscience and I would like to use some antibodies, especially in Western Blots, but I could do other assays: - alpha7 nicotinic cholinergic receptor; - parvalbumin; - calbindin D28K; The preferred reactivity is anti-human and anti-mouse. Do you have these antibodies? Are they in stock? And what about the prizes: How much are they? How much are the shipping costs? Could you make me any offer?

A: We currently have three alpha7 nicotinic cholingergic receptor antibodies available for purchase. Please see this list for our Parvalbumin antibodies. None are currently validated in mouse. I would like to introduce you to our Innovators Reward Program. We have an excelent calbinin D28K antibody that has been validated for use in human and mouse and WB. The pricing and availability of our products depends on your country.

Parvalbumin Antibody

Novus Biologicals | Catalog # NB100-1541

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Product Documents

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Rat

Applications

Western Blot, Peptide ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

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Product Specifications

Immunogen

Peptide with sequence C-GVDEFSTLVAES corresponding to C-Terminus according to NP_002845.1.

Epitope

C Terminus

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Parvalbumin Antibody

Western Blot: Parvalbumin Antibody [NB100-1541]

Western Blot: Parvalbumin Antibody [NB100-1541] - (0.01ug/ml) staining of Human Cerebellum (A) lysate + peptide (B) and (0.3ug/ml) Rat Brain (C) lysate + peptide (D) (35ug protein in RIPA buffer). Detected by chemiluminescence.

Flow Cytometry: Parvalbumin Antibody [NB100-1541]

Flow Cytometry: Parvalbumin Antibody [NB100-1541] - Analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Applications for Parvalbumin Antibody

Application

Recommended Usage

Flow Cytometry

10 ug/ml

Peptide ELISA

Detection limit 1:64000

Western Blot

0.01-0.3 ug/ml

Application Notes

Western blot: Approx 15kDa band observed in Human Cerebellum lysates and approx 16kDa in Rat Brain lysates (calculated MW of 12.1kDa according to Human NP_002845.1 and 11.9kDa according to Rat NP_071944.1). These molecular weights are observed by other sources and were successfully blocked by incubation with the immunizing peptide.
Flow Cytometry: Flow cytometric analysis of Kelly cells.

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Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Parvalbumin

The protein encoded by this gene is a high affinity calcium ion-binding protein that is structurally and functionally similar to calmodulin and troponin C. The encoded protein is thought to be involved in muscle relaxation.

Alternate Names

D22S749, MGC116759, parvalbumin, parvalbumin alpha, PV

Entrez Gene IDs

5816 (Human); 25269 (Rat)

Gene Symbol

PVALB

UniProt

NP_002845.1 (Human)

Additional Parvalbumin Products

Product Documents for Parvalbumin Antibody

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Certificate of Analysis

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Product Specific Notices for Parvalbumin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Parvalbumin Antibody

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