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Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 3C12
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Product Specifications
Immunogen
Purified recombinant fragment of human Pax4 expressed in E. Coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Pax4 Antibody (3C12)
Western Blot: Pax4 Antibody (3C12)BSA Free [NBP2-37644]
Western Blot: Pax4 Antibody (3C12) [NBP2-37644] - Western blot analysis using PAX4 mAb against HEK293 (1) and PAX4(AA: 105-232)-hIgGFc transfected HEK293 (2) cell lysate.ELISA: Pax4 Antibody (3C12) - BSA Free [NBP2-37644]
ELISA: Pax4 Antibody (3C12) [NBP2-37644] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);Applications for Pax4 Antibody (3C12)
Application
Recommended Usage
ELISA
1:10000
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Ascites
Formulation
Ascites
Preservative
0.03% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Pax4
Long Name
Paired Box Gene 4
Alternate Names
KPD, MGC129960, MODY9paired box protein Pax-4, paired box 4, paired box gene 4, paired domain gene 4
Gene Symbol
PAX4
Additional Pax4 Products
Product Documents for Pax4 Antibody (3C12)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Pax4 Antibody (3C12)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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