Pax7 Antibody (3C9) - Azide and BSA Free
Novus Biologicals | Catalog # H00005081-M03
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA, Sandwich ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 3C9
Format
Azide and BSA Free
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Product Specifications
Immunogen
PAX7 (NP_002575.1, 411 a.a. ~ 520 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GGLDSATSISASCSQRADSIKPGDSLPTSQAYCPPTYSTTGYSVDPVAGYQYGQYGQSECLVPWASPVPIPSPTPRASCLFMESYKVVSGWGMSISQMEKLKSSQMEQFT
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Specificity
PAX7 - paired box gene 7
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Pax7 Antibody (3C9) - Azide and BSA Free
Western Blot: Pax7 Antibody (3C9) [H00005081-M03]
Western Blot: Pax7 Antibody (3C9) [H00005081-M03] - Analysis of PAX7 expression in Raw 264.7 (Cat # L024V1).Western Blot: Pax7 Antibody (3C9) [H00005081-M03]
Western Blot: Pax7 Antibody (3C9) [H00005081-M03] - Analysis of PAX7 expression in HeLa (Cat # L013V1).Western Blot: Pax7 Antibody (3C9) [H00005081-M03]
Western Blot: Pax7 Antibody (3C9) [H00005081-M03] - Analysis of PAX7 expression in PC-12 (Cat # L012V1).
Sandwich ELISA: Pax7 Antibody (3C9) [H00005081-M03] - Detection limit for recombinant GST tagged PAX7 is approximately 3ng/ml as a capture antibody.
Applications for Pax7 Antibody (3C9) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Pax7
Long Name
Paired Box Gene 7
Alternate Names
HUP1, RMS2
Entrez Gene IDs
5081 (Human)
Gene Symbol
PAX7
OMIM
167410 (Human)
UniProt
Additional Pax7 Products
Product Documents for Pax7 Antibody (3C9) - Azide and BSA Free
Product Specific Notices for Pax7 Antibody (3C9) - Azide and BSA Free
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
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- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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