Paxillin [p Ser273] Antibody

Novus Biologicals | Catalog # NBP3-13099

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Chicken (100%), Rat (100%), Xenopus (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Paxillin Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser273 of human Paxillin. The exact sequence is proprietary.

Modification

p Ser 273

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Paxillin [p Ser273] Antibody

Western Blot: Paxillin [p Ser273] Antibody [NBP3-13099]

Western Blot: Paxillin [p Ser273] Antibody [NBP3-13099]

Western Blot: Paxillin [p Ser273] Antibody [NBP3-13099] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Ser273) antibody (NBP3-13099) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: Paxillin [p Ser273] Antibody [NBP3-13099]

Western Blot: Paxillin [p Ser273] Antibody [NBP3-13099]

Western Blot: Paxillin [p Ser273] Antibody [NBP3-13099] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Ser273) antibody (NBP3-13099) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Applications for Paxillin [p Ser273] Antibody

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Paxillin

Paxillin is a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion) (1). Paxillin plays a significant role in integrin signaling and the tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization (2). Paxillin is phosphorylated by focal adhesion kinase (FAK) at Tyr118 (3). It has been found that phosphorylation of paxillin at both Tyr118 and Tyr31 is essential for MM1 cancer cell migration (4).

Alternate Names

PXN

Gene Symbol

PXN

Additional Paxillin Products

Product Documents for Paxillin [p Ser273] Antibody

Certificate of Analysis

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Product Specific Notices for Paxillin [p Ser273] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

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