pCL-Ampho Retrovirus Packaging Vector

Novus Biologicals | Catalog # NBP2-29541

Novus Biologicals
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Key Product Details

Species

Hamster (Negative), Mammal

Applications

Immunocytochemistry/ Immunofluorescence

Product Specifications

Specificity

Ecotropic (MoMuLV) for mouse and rat cells only (not human)
Amphotropic (from 4070A MuLV) for most mammalian cells (but not hamster)
10A1 (MuLV) for most mammalian cells (including hamster)

Applications

Immunocytochemistry/ Immunofluorescence (reported in scientific literature (PMID 34082772))
Retroviral Production (reported in scientific literature (PMID 24311986))

Application Notes

For amplification, transform a suitable E. coli host such as HB101 or DH-5alpha and plate on LB plates containing 50-ug/ml ampicillin. For plasmid DNA preparation start culture from a single colony in LB medium containing 100 ug/ml ampicillin.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 24311986).

Formulation, Preparation, and Storage

Formulation

10 ug in 20 ul sterile water.

Concentration

Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: pCL-Ampho Retrovirus Packaging Vector

The pCL-Ampho packaging vector is a part of the RetroMax expression system and has been designed to maximize recombinant-retrovirus titers in a simple, efficient, and flexible experimental system. By introducing a retroviral vector into a cell expressing retroviral proteins, retroviral particles (virions) are shed into the culture medium at the rate of 1 infectious particle/cell/day. Retrovirus tropism is determined at three levels. The first is simply a function of viral envelope protein, gp70. The envelope determines which cells the virus will enter. gp70 comes in three different flavors for gene therapy. The plasmid DNA can be amplified by transforming bacteria and growing them on LB/ampicillin (100 ug/ml) media.

Alternate Names

pCLAmpho, pCL-Ampho, pcl-ampho packaging vector, pCL-Ampho Retrovirus

Additional pCL-Ampho Retrovirus Packaging Vector Products

Product Documents for pCL-Ampho Retrovirus Packaging Vector

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for pCL-Ampho Retrovirus Packaging Vector

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

Citations for pCL-Ampho Retrovirus Packaging Vector

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Protocols

View specific protocols for pCL-Ampho Retrovirus Packaging Vector (NBP2-29541):


Day 0 Seed 293 cells and grow overnight.
Day 1 Transfect with retroviral vector containing gene of interest and an appropriate packaging vector.
Day 2 Replace medium.
Day 3 Harvest virus-containing supernatant. Virus may be stored at - 70C at this stage. Infect target cells,either for titer determination or for gene expression.
Day 4 Split infected target cells and grow for selecting stable virus producing cell lines. For transient expression experiments, you may harvest the cells at this stage.
Day 5 Start selection by replacing the medium with G418 containing medium.
Day 9 Change medium and continue selection.
Day 14 Count antibiotic resistant colonies and calculate titer.
Note: If you are using retroviral expression system for the first time, we strongly recommend using the LacZ control plasmid included in the kit. The B-galactosidase expression can be monitored using B-gal staining kit or any other standard protocol.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for pCL-Ampho Retrovirus Packaging Vector

Showing  1 - 4 of 4 FAQs Showing All

  • Q: I am having a retroviral system in place which allows me to infect mouse cell lines. The vectors I have are MIG.R (with my gene of interest), pCL-ECO and VSV-g. I know that my pCL-ECO vector is only suitable for infecting mouse cells. My question is: Is it possible to use my MIG.R and VSV-g vectors, and exchange the mouse specific pCL-ECO with pCL-Ampho or pCL-10A1 packaging vector which you both offer for purchase (Cat Numbers are 10045P, 10046P, 10047P). My aim is to infect human cell lines with my gene of interest.

    A:

    These vectors, while also sold separately, are components of the RetroMax system 10040K. The vectors are designed to be used as described in the 10040K manual. Yes, you are correct, pCL-ECO is for mouse and rat, and not for human. We have not evaluated the Retromax system with the MIG.R and VSV-g vectors, and can not promise they would be compatible with your vectors. The Retromax packaging vectors are designed to be used with the expression vectors in the Retromax Kit. However, the packaging vectors are widely published and you may be able to find some information in the literature relevant to your model system (search scholar.google.com and keywords with Retromax and vector name, or keys words with packaging vector name and expression vector name ).

  • Q: I was curious if you had a map for the pcl-ampho vector?

    A: Unfortunately, we do not have a pCL-Ampho Vector Map in our records. This product was originally developed by Imgenex, a company we acquired at the beginning of last year, and it does not appear that the vector maps were supplied to us. We do have several publications referencing this product and you may find some of those publications useful: click on Reviews & Publications tab at NBP2-29541.

  • Q: NBP2-29541 what is the sequence?

    A:

    As stated under "Notes," the sequence of NBP2-29541 is not available.
    You can find out more information about this plasmid in the manual, including a plasmid map on page 22.  The map is for pCl-ECO but the only difference between the Eco and Ampho plasmids is the envelope gene.

  • Q: We would like to make sure that your pCL-Ampho Retrovirus Packaging Vector is compatible with the MSCV (Murine Stem Cell Virus) Retroviral Expression System.

    A: The pCL-Ampho Retrovirus Packaging Vector NBP2-29541 should be compatible with a MSCV expression system. Please see the following publication that utilized MSCV2.2 with this packaging vector: Publication link

  • Q: I am having a retroviral system in place which allows me to infect mouse cell lines. The vectors I have are MIG.R (with my gene of interest), pCL-ECO and VSV-g. I know that my pCL-ECO vector is only suitable for infecting mouse cells. My question is: Is it possible to use my MIG.R and VSV-g vectors, and exchange the mouse specific pCL-ECO with pCL-Ampho or pCL-10A1 packaging vector which you both offer for purchase (Cat Numbers are 10045P, 10046P, 10047P). My aim is to infect human cell lines with my gene of interest.

    A:

    These vectors, while also sold separately, are components of the RetroMax system 10040K. The vectors are designed to be used as described in the 10040K manual. Yes, you are correct, pCL-ECO is for mouse and rat, and not for human. We have not evaluated the Retromax system with the MIG.R and VSV-g vectors, and can not promise they would be compatible with your vectors. The Retromax packaging vectors are designed to be used with the expression vectors in the Retromax Kit. However, the packaging vectors are widely published and you may be able to find some information in the literature relevant to your model system (search scholar.google.com and keywords with Retromax and vector name, or keys words with packaging vector name and expression vector name ).

  • Q: I was curious if you had a map for the pcl-ampho vector?

    A: Unfortunately, we do not have a pCL-Ampho Vector Map in our records. This product was originally developed by Imgenex, a company we acquired at the beginning of last year, and it does not appear that the vector maps were supplied to us. We do have several publications referencing this product and you may find some of those publications useful: click on Reviews & Publications tab at NBP2-29541.

  • Q: NBP2-29541 what is the sequence?

    A:

    As stated under "Notes," the sequence of NBP2-29541 is not available.
    You can find out more information about this plasmid in the manual, including a plasmid map on page 22.  The map is for pCl-ECO but the only difference between the Eco and Ampho plasmids is the envelope gene.

  • Q: We would like to make sure that your pCL-Ampho Retrovirus Packaging Vector is compatible with the MSCV (Murine Stem Cell Virus) Retroviral Expression System.

    A: The pCL-Ampho Retrovirus Packaging Vector NBP2-29541 should be compatible with a MSCV expression system. Please see the following publication that utilized MSCV2.2 with this packaging vector: Publication link

  • Q: I am having a retroviral system in place which allows me to infect mouse cell lines. The vectors I have are MIG.R (with my gene of interest), pCL-ECO and VSV-g. I know that my pCL-ECO vector is only suitable for infecting mouse cells. My question is: Is it possible to use my MIG.R and VSV-g vectors, and exchange the mouse specific pCL-ECO with pCL-Ampho or pCL-10A1 packaging vector which you both offer for purchase (Cat Numbers are 10045P, 10046P, 10047P). My aim is to infect human cell lines with my gene of interest.

    A:

    These vectors, while also sold separately, are components of the RetroMax system 10040K. The vectors are designed to be used as described in the 10040K manual. Yes, you are correct, pCL-ECO is for mouse and rat, and not for human. We have not evaluated the Retromax system with the MIG.R and VSV-g vectors, and can not promise they would be compatible with your vectors. The Retromax packaging vectors are designed to be used with the expression vectors in the Retromax Kit. However, the packaging vectors are widely published and you may be able to find some information in the literature relevant to your model system (search scholar.google.com and keywords with Retromax and vector name, or keys words with packaging vector name and expression vector name ).

  • Q: I was curious if you had a map for the pcl-ampho vector?

    A: Unfortunately, we do not have a pCL-Ampho Vector Map in our records. This product was originally developed by Imgenex, a company we acquired at the beginning of last year, and it does not appear that the vector maps were supplied to us. We do have several publications referencing this product and you may find some of those publications useful: click on Reviews & Publications tab at NBP2-29541.

  • Q: NBP2-29541 what is the sequence?

    A:

    As stated under "Notes," the sequence of NBP2-29541 is not available.
    You can find out more information about this plasmid in the manual, including a plasmid map on page 22.  The map is for pCl-ECO but the only difference between the Eco and Ampho plasmids is the envelope gene.

  • Q: We would like to make sure that your pCL-Ampho Retrovirus Packaging Vector is compatible with the MSCV (Murine Stem Cell Virus) Retroviral Expression System.

    A: The pCL-Ampho Retrovirus Packaging Vector NBP2-29541 should be compatible with a MSCV expression system. Please see the following publication that utilized MSCV2.2 with this packaging vector: Publication link

  • Q: I am having a retroviral system in place which allows me to infect mouse cell lines. The vectors I have are MIG.R (with my gene of interest), pCL-ECO and VSV-g. I know that my pCL-ECO vector is only suitable for infecting mouse cells. My question is: Is it possible to use my MIG.R and VSV-g vectors, and exchange the mouse specific pCL-ECO with pCL-Ampho or pCL-10A1 packaging vector which you both offer for purchase (Cat Numbers are 10045P, 10046P, 10047P). My aim is to infect human cell lines with my gene of interest.

    A:

    These vectors, while also sold separately, are components of the RetroMax system 10040K. The vectors are designed to be used as described in the 10040K manual. Yes, you are correct, pCL-ECO is for mouse and rat, and not for human. We have not evaluated the Retromax system with the MIG.R and VSV-g vectors, and can not promise they would be compatible with your vectors. The Retromax packaging vectors are designed to be used with the expression vectors in the Retromax Kit. However, the packaging vectors are widely published and you may be able to find some information in the literature relevant to your model system (search scholar.google.com and keywords with Retromax and vector name, or keys words with packaging vector name and expression vector name ).

  • Q: I was curious if you had a map for the pcl-ampho vector?

    A: Unfortunately, we do not have a pCL-Ampho Vector Map in our records. This product was originally developed by Imgenex, a company we acquired at the beginning of last year, and it does not appear that the vector maps were supplied to us. We do have several publications referencing this product and you may find some of those publications useful: click on Reviews & Publications tab at NBP2-29541.

  • Q: NBP2-29541 what is the sequence?

    A:

    As stated under "Notes," the sequence of NBP2-29541 is not available.
    You can find out more information about this plasmid in the manual, including a plasmid map on page 22.  The map is for pCl-ECO but the only difference between the Eco and Ampho plasmids is the envelope gene.

  • Q: We would like to make sure that your pCL-Ampho Retrovirus Packaging Vector is compatible with the MSCV (Murine Stem Cell Virus) Retroviral Expression System.

    A: The pCL-Ampho Retrovirus Packaging Vector NBP2-29541 should be compatible with a MSCV expression system. Please see the following publication that utilized MSCV2.2 with this packaging vector: Publication link

Showing  1 - 4 of 4 FAQs Showing All
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