PCNA Antibody (OTI3D6)

Novus Biologicals | Catalog # NBP2-46342

Novus Biologicals

Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI3D6
View all PCNA Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PCNA (NP_002583) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

28.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PCNA Antibody (OTI3D6)

Western Blot: PCNA Antibody (OTI3D6) [NBP2-46342]

Western Blot: PCNA Antibody (OTI3D6) [NBP2-46342]

Western Blot: PCNA Antibody (3D6) [NBP2-46342] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PCNA.
Immunohistochemistry: PCNA Antibody (OTI3D6) [NBP2-46342]

Immunohistochemistry: PCNA Antibody (OTI3D6) [NBP2-46342]

Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: PCNA Antibody (OTI3D6) [NBP2-46342]

Western Blot: PCNA Antibody (OTI3D6) [NBP2-46342]

Western Blot: PCNA Antibody (3D6) [NBP2-46342] - Analysis of extracts (35ug) from 9 different cell lines by using PCNA monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human). Dilution: 1:2000
Immunohistochemistry: PCNA Antibody (OTI3D6) [NBP2-46342]

Immunohistochemistry: PCNA Antibody (OTI3D6) [NBP2-46342]

Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: PCNA Antibody (OTI3D6) [NBP2-46342]

Immunohistochemistry: PCNA Antibody (OTI3D6) [NBP2-46342]

Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: PCNA Antibody (OTI3D6) [NBP2-46342]

Immunohistochemistry: PCNA Antibody (OTI3D6) [NBP2-46342]

Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: PCNA Antibody (OTI3D6) [NBP2-46342]

Immunohistochemistry: PCNA Antibody (OTI3D6) [NBP2-46342]

Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: PCNA Antibody (OTI3D6) [NBP2-46342]

Immunohistochemistry: PCNA Antibody (OTI3D6) [NBP2-46342]

Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: PCNA Antibody (OTI3D6) [NBP2-46342]

Immunohistochemistry: PCNA Antibody (OTI3D6) [NBP2-46342]

Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: PCNA Antibody (OTI3D6) [NBP2-46342]

Immunohistochemistry: PCNA Antibody (OTI3D6) [NBP2-46342]

Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: PCNA Antibody (OTI3D6) [NBP2-46342]

Immunohistochemistry: PCNA Antibody (OTI3D6) [NBP2-46342]

Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: PCNA Antibody (OTI3D6) [NBP2-46342]

Immunohistochemistry: PCNA Antibody (OTI3D6) [NBP2-46342]

Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human Ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: PCNA Antibody (OTI3D6) [NBP2-46342]

Immunohistochemistry: PCNA Antibody (OTI3D6) [NBP2-46342]

Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: PCNA Antibody (OTI3D6) [NBP2-46342]

Immunohistochemistry: PCNA Antibody (OTI3D6) [NBP2-46342]

Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: PCNA Antibody (OTI3D6) [NBP2-46342]

Immunohistochemistry: PCNA Antibody (OTI3D6) [NBP2-46342]

Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: PCNA Antibody (OTI3D6) [NBP2-46342]

Immunohistochemistry: PCNA Antibody (OTI3D6) [NBP2-46342]

Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: PCNA Antibody (OTI3D6) [NBP2-46342]

Immunohistochemistry: PCNA Antibody (OTI3D6) [NBP2-46342]

Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: PCNA Antibody (OTI3D6) [NBP2-46342]

Immunohistochemistry: PCNA Antibody (OTI3D6) [NBP2-46342]

Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: PCNA Antibody (OTI3D6) [NBP2-46342]

Immunohistochemistry: PCNA Antibody (OTI3D6) [NBP2-46342]

Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: PCNA Antibody (OTI3D6) [NBP2-46342]

Immunohistochemistry: PCNA Antibody (OTI3D6) [NBP2-46342]

Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: PCNA Antibody (OTI3D6) [NBP2-46342]

Immunohistochemistry: PCNA Antibody (OTI3D6) [NBP2-46342]

Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: PCNA Antibody (OTI3D6) [NBP2-46342]

Immunohistochemistry: PCNA Antibody (OTI3D6) [NBP2-46342]

Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: PCNA Antibody (OTI3D6) [NBP2-46342]

Immunohistochemistry: PCNA Antibody (OTI3D6) [NBP2-46342]

Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Applications for PCNA Antibody (OTI3D6)

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PCNA

Proliferating Cell Nuclear Antigen (PCNA) is a DNA polymerase that is essential for DNA replication, excision and mismatch repair pathways. PCNA is a "DNA clamp" which binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumor tissue (i.e. relevant to prognosis). PCNA antibodies are also useful tools for DNA repair and cancer research.

Long Name

Proliferating Cell Nuclear Antigen

Alternate Names

cyclin, DNA polymerase delta auxiliary protein, MGC8367, proliferating cell nuclear antigen

Gene Symbol

PCNA

Additional PCNA Products

Product Documents for PCNA Antibody (OTI3D6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for PCNA Antibody (OTI3D6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for PCNA Antibody (OTI3D6)

There are currently no reviews for this product. Be the first to review PCNA Antibody (OTI3D6) and earn rewards!

Have you used PCNA Antibody (OTI3D6)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies