PD-1 Antibody (EH12.2H7) - Low Endotoxin

Novus Biologicals | Catalog # NBP3-09097

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry, Functional

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # EH12.2H7

Format

Low Endotoxin
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Product Specifications

Immunogen

Immunogen from PD-1

Specificity

The mouse monoclonal antibody EH12.2H7 recognizes an extracellular epitope of CD279 / PD-1 (programmed cell death 1), a 55 kDa type I transmembrane protein expressed above all during T cell development, on activated T cells, activated B cells, and activated monocytes.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Endotoxin Level

Endotoxin level is less than 0.01 EU/ug of the protein, as determined by the LAL test.

Scientific Data Images for PD-1 Antibody (EH12.2H7) - Low Endotoxin

Flow Cytometry: PD-1 Antibody (EH12.2H7) - Low Endotoxin, Azide and BSA Free [NBP3-09097]

Flow Cytometry: PD-1 Antibody (EH12.2H7) - Low Endotoxin, Azide and BSA Free [NBP3-09097]

Flow Cytometry: PD-1 Antibody (EH12.2H7) - Low Endotoxin, Azide and BSA Free [NBP3-09097] - Separation of human CD279 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD279 (EH12.2H7) purified antibody (low endotoxin, concentration in sample 6 ug/ml) GAM APC.
Flow Cytometry: PD-1 Antibody (EH12.2H7) - Low Endotoxin, Azide and BSA Free [NBP3-09097]

Flow Cytometry: PD-1 Antibody (EH12.2H7) - Low Endotoxin, Azide and BSA Free [NBP3-09097]

Flow Cytometry: PD-1 Antibody (EH12.2H7) - Low Endotoxin, Azide and BSA Free [NBP3-09097] - Surface staining pattern of human peripheral blood stained using anti-human CD279 (EH12.2H7) purified antibody (low endotoxin, concentration in sample 6 ug/ml) GAM APC.

Applications for PD-1 Antibody (EH12.2H7) - Low Endotoxin

Application
Recommended Usage

Flow Cytometry

1-4 ug/ml

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

Low Endotoxin

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PD-1

PD1 is an inhibitory cell membrane surface receptor from the immunoglobulin superfamily. Involved in the regulation of T cell function during immunity and tolerance, PD1 inhibits T cell effector functions upon ligand binding. The protein is expressed in pro-B-cells and is thought to play a role in their differentiation as well. Studies have also suggested that this gene product may also contribute to the prevention of autoimmune diseases.

Long Name

Programmed Death-1

Alternate Names

CD279, PD1, PDCD1, SLEB2

Gene Symbol

PDCD1

Additional PD-1 Products

Product Documents for PD-1 Antibody (EH12.2H7) - Low Endotoxin

Certificate of Analysis

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Product Specific Notices for PD-1 Antibody (EH12.2H7) - Low Endotoxin

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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