PD-L2/B7-DC/PDCD1LG2 Antibody (24F.10C12) - BSA Free

Novus Biologicals | Catalog # NBP3-09141

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 24F.10C12

Format

BSA Free
View all PD-L2/B7-DC Primary Antibodies »

Product Specifications

Immunogen

human PD-L2/B7-DC/PDCD1LG2

Specificity

The mouse monoclonal antibody 24F.10C12 recognizes an extracellular epitope of CD273 / PD-L2 (also known as B7-DC), a 25 kDa type I transmembrane protein expressed by dendritic cells, activated monocytes and T cells, heart, first trimester placenta, lung and liver, as well as in Hodgkin's lymphoma cells and primary mediastinal B cell lymphoma (PMBL).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for PD-L2/B7-DC/PDCD1LG2 Antibody (24F.10C12) - BSA Free

PD-L2/B7-DC/PDCD1LG2 Antibody (24F.10C12) - BSA Free Flow Cytometry: PD-L2/B7-DC/PDCD1LG2 Antibody (24F.10C12) - BSA Free [NBP3-09141]

Flow Cytometry: PD-L2/B7-DC/PDCD1LG2 Antibody (24F.10C12) - BSA Free [NBP3-09141]

Separation of human CD11c positive HLA-DR positive cells (red-filled) from CD11c negative HLA-DR negative cells (black-dashed) in Analysis (surface staining) of human stimulated (GM-CSF + IL-4) monocytes stained using anti-human CD273 (24F.10C12) purified antibody (concentration in sample 0.6 ug/ml) GAM APC.

Applications for PD-L2/B7-DC/PDCD1LG2 Antibody (24F.10C12) - BSA Free

Application
Recommended Usage

Flow Cytometry

1-4 ug/ml

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PD-L2/B7-DC

B7-DC is also called programmed death ligand 2 (PDL2). It has recently been clustered as CD273. This ligand is a 42 kD member of the immunoglobulin receptor superfamily expressed on a subset of dendritic cells, liver and a small subset of macrophages as well as a few transformed cell lines. B7-DC (CD273) has been reported to be stimulatory on dendritic cells when cross-linked and to inhibit T cell activation upon engaging the PD-1 receptor. B7-DC (CD273) has also been reported to bind to an alternative receptor and to mediate T cell activation through such non-PD1 mediated interactions.

Long Name

Programmed Death-1 Ligand-2

Alternate Names

B7-DC, Butyrophilin-like Protein, CD273, PDCD1LG2, PDL2

Gene Symbol

PDCD1LG2

Additional PD-L2/B7-DC Products

Product Documents for PD-L2/B7-DC/PDCD1LG2 Antibody (24F.10C12) - BSA Free

Certificate of Analysis

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Product Specific Notices for PD-L2/B7-DC/PDCD1LG2 Antibody (24F.10C12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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