PDK4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-54723

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Knockdown Validated

Cited:

Immunohistochemistry-Paraffin, Western Blot, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PDK4 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptides corresponding to PDK4(pyruvate dehydrogenase kinase, isozyme 4) The peptide sequence was selected from the N terminal of PDK4. Peptide sequence MKAARFVLRSAGSLNGAGLVPREVEHFSRYSPSPLSMKQLLDFGSENACE. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for PDK4 Antibody - BSA Free

Western Blot: PDK4 Antibody [NBP1-54723]

Western Blot: PDK4 Antibody [NBP1-54723]

Western Blot: PDK4 Antibody [NBP1-54723] - Titration: 0.2-1 ug/ml Positive Control: Transfected 293T.
Immunohistochemistry-Paraffin: PDK4 Antibody [NBP1-54723]

Immunohistochemistry-Paraffin: PDK4 Antibody [NBP1-54723]

Immunohistochemistry-Paraffin: PDK4 Antibody [NBP1-54723] - Human Muscle Tissue, Skeletal muscle cells (Indicated with Arrows) 4-8ug/ml.
Western Blot: PDK4 Antibody [NBP1-54723]

Western Blot: PDK4 Antibody [NBP1-54723]

Western Blot: PDK4 Antibody [NBP1-54723] - Sample Type: Huh7 HepG2 (50ug) Primary Antibody Dilution: 1:500 Image Submitted By: Partha Kasturi University of Kansas Medical Center.
PDK4 Antibody - BSA Free

Western Blot: PDK4 Antibody - BSA Free [NBP1-54723] -

CCM treatment regulates the key glucose metabolism factors in rabbits with HF. (a) Western blot analysis of the expression of GLUT4. Densitometric analysis is shown in the bar graph. (b) Western blot analysis of the expressions of HK2, PFK6, PDH, and PDK4. Densitometric analysis is shown in the bar graph. Data are expressed as mean values +/- SD (*P < 0.05 compared with the sham group; #P < 0.05 compared with HF group). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35799598), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for PDK4 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PDK4

PDK4 is a member of the PDK/BCKDK protein kinase family and is a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism.This gene is a member of the PDK/BCKDK protein kinase family and encodes a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism. Expression of this gene is regulated by glucocorticoids, retinoic acid and insulin.

Alternate Names

[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial, EC 2.7.11, EC 2.7.11.2, FLJ40832, Pyruvate dehydrogenase kinase isoform 4, pyruvate dehydrogenase kinase, isoenzyme 4, pyruvate dehydrogenase kinase, isozyme 4, pyruvate dehydrogenase, lipoamide, kinase isozyme 4, mitochondrial

Gene Symbol

PDK4

UniProt

Q16654 (Human)

Additional PDK4 Products

Product Documents for PDK4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PDK4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PDK4 Antibody - BSA Free

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Protocols

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FAQs for PDK4 Antibody - BSA Free

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  • Q: Which PDK4 antibody do you recommend for immunoblot? I'm working with human cell lines.

    A: NBP1-07047 and NBP1-07049 are our best selling PDK4 antibodies that will meet your mentioned criteria i.e. Immunoblot/Western Blot in Human samples. However, all of our products are 100% guaranteed and you may find a complete list of PDK4 antibodies by searching PDK4 on our website and narrowing your results using the filters on the left-hand side of the screen.

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