PDLIM1 Antibody (5X10L2)

Novus Biologicals | Catalog # NBP3-15689

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5X10L2 expressed in HEK293
View all PDLIM1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human PDLIM1 (O00151). LQEKQELNEPPKQSTSFLVLQEILESEEKGDPNKPSGFRSVKAPVTKVAASIGNAQKLPMCDKCGTGIVGVFVKLRDRHRHPECYVCTDCGTNLKQKGHFF

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PDLIM1 Antibody (5X10L2)

Western Blot: PDLIM1 Antibody (5X10L2) [NBP3-15689]

Western Blot: PDLIM1 Antibody (5X10L2) [NBP3-15689]

Western Blot: PDLIM1 Antibody (5X10L2) [NBP3-15689] - Western blot analysis of extracts of various cell lines, using PDLIM1 antibody (NBP3-15689) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunocytochemistry/ Immunofluorescence: PDLIM1 Antibody (5X10L2) [NBP3-15689]

Immunocytochemistry/ Immunofluorescence: PDLIM1 Antibody (5X10L2) [NBP3-15689]

Immunocytochemistry/Immunofluorescence: PDLIM1 Antibody (5X10L2) [NBP3-15689] - Immunofluorescence analysis of NIH/3T3 cells using PDLIM1 Rabbit mAb (NBP3-15689) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blot: PDLIM1 Antibody (5X10L2) [NBP3-15689]

Western Blot: PDLIM1 Antibody (5X10L2) [NBP3-15689]

Western Blot: PDLIM1 Antibody (5X10L2) [NBP3-15689] - Western blot analysis of extracts of Mouse thymus, using PDLIM1 antibody (NBP3-15689) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Applications for PDLIM1 Antibody (5X10L2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PDLIM1

CLP36 is a PDZ-domain and LIM-domain containing protein that associates with actin filaments and stress fibers via alpha-actinin. CLP36 has been show to be critical to stress fiber formation and focal adhesions. Alternative names for CLP36 include Elfin, C-terminal LIM domain protein 1, PDZ and LIM domain protein 1, CLIM1, and PDLIM1.

Alternate Names

CLIM1carboxyl terminal LIM domain protein 1, CLP-36, CLP36carboxy terminal LIM domain protein 1, C-terminal LIM domain protein 1, elfin, enigma, hCLIM1, LIM domain protein CLP-36, PDZ and LIM domain 1, PDZ and LIM domain 1 (elfin), PDZ and LIM domain protein 1

Gene Symbol

PDLIM1

Additional PDLIM1 Products

Product Documents for PDLIM1 Antibody (5X10L2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for PDLIM1 Antibody (5X10L2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for PDLIM1 Antibody (5X10L2)

There are currently no reviews for this product. Be the first to review PDLIM1 Antibody (5X10L2) and earn rewards!

Have you used PDLIM1 Antibody (5X10L2)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies