PDX-1/IPF1 Antibody (5A5) - BSA Free

Novus Biologicals | Catalog # NBP2-37506

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5A5

Format

BSA Free
View all PDX-1/IPF1 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human PDX-1/IPF1 expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

30.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PDX-1/IPF1 Antibody (5A5) - BSA Free

Western Blot: PDX-1/IPF1 Antibody (5A5)BSA Free [NBP2-37506]

Western Blot: PDX-1/IPF1 Antibody (5A5)BSA Free [NBP2-37506]

Western Blot: PDX-1/IPF1 Antibody (5A5) [NBP2-37506] - Western blot analysis using PDX1 mAb against HEK293 (1) and PDX1 (AA: 39-283)-hIgGFc transfected HEK293 (2) cell lysate.
Flow Cytometry: PDX-1/IPF1 Antibody (5A5) - BSA Free [NBP2-37506]

Flow Cytometry: PDX-1/IPF1 Antibody (5A5) - BSA Free [NBP2-37506]

Flow Cytometry: PDX-1/IPF1 Antibody (5A5) [NBP2-37506] - Flow cytometric analysis of Jurkat cells using PDX1 mouse mAb (green) and negative control (red).
Western Blot: PDX-1/IPF1 Antibody (5A5)BSA Free [NBP2-37506]

Western Blot: PDX-1/IPF1 Antibody (5A5)BSA Free [NBP2-37506]

Western Blot: PDX-1/IPF1 Antibody (5A5) [NBP2-37506] - Western blot analysis using PDX1 mAb against human PDX1 (AA: 39-283) recombinant protein. (Expected MW is 52 kDa)
ELISA: PDX-1/IPF1 Antibody (5A5) - BSA Free [NBP2-37506]

ELISA: PDX-1/IPF1 Antibody (5A5) - BSA Free [NBP2-37506]

ELISA: PDX-1/IPF1 Antibody (5A5) [NBP2-37506] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for PDX-1/IPF1 Antibody (5A5) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PDX-1/IPF1

PDX1 (Pancrease/duodenum homeobox 1, Insulin promoter factor 1, IPF1, Islet/duodenum homeobox 1, IDX1, Somatatostatin transactivating factor 1, STF1, Insulin upstream factor 1, UUF1, Glucose-sensitive factor, GSF) activates transcription of the insulin and somatostain genes. PDX1 is required for maintaining the hormone-producing phenotype of the beta-cell.

Long Name

Pancreas/Duodenum Homeobox-1

Alternate Names

IDX1, IPF1

Gene Symbol

PDX1

Additional PDX-1/IPF1 Products

Product Documents for PDX-1/IPF1 Antibody (5A5) - BSA Free

Certificate of Analysis

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Product Specific Notices for PDX-1/IPF1 Antibody (5A5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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