Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4D117

Format

BSA Free
View all PEA-15 Primary Antibodies »

Product Specifications

Immunogen

Full length human PEA-15 as expressed in and purified from E. coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse PEA-15 Antibody (4D117) - BSA Free (NBP3-05543) is a monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for PEA-15 Antibody (4D117) - BSA Free

Western Blot: PEA-15 Antibody [NBP3-05543]

Western Blot: PEA-15 Antibody [NBP3-05543]

Western Blot: PEA-15 Antibody [NBP3-05543] - Western blot analysis of different tissue lysates using PEA-15 Antibody at a dilution of 1:1,000 in green: [1] protein standard (red), [2] rat whole brain, [3] rat cerebellum, [4] mouse whole brain, [5] cow cortex and [6] cow cerebellum. The strong band at about 15kDa corresponds to the PEA-15 protein.
Immunocytochemistry/ Immunofluorescence: PEA-15 Antibody [NBP3-05543]

Immunocytochemistry/ Immunofluorescence: PEA-15 Antibody [NBP3-05543]

Immunocytochemistry/Immunofluorescence: PEA-15 Antibody [NBP3-05543] - Immunofluorescent analysis of rat brain section stained with PEA-15 Antibody at a dilution of 1:1,000 in red, and costained with chicken pAb to MAP2 at a dilution of 1:5,000 in green. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The PEA-15 antibody labels the cytoplasm of certain presumably neuronal cells which are not labelled by the astrocyte specific GFAP antibody.

Applications for PEA-15 Antibody (4D117) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000-2000

Western Blot

1:1000-1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50% PBS, 50% glycerol

Format

BSA Free

Preservative

5mM Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.

Background: PEA-15

PEA-15 (phosphoprotein enriched in astrocytes) exists in an non-phosphorylated form (N), and two phosphorylated forms, Pa and Pb. PEA-15 is an endogenous substrate for PKC, which mediates the transition from Pa to Pb. The level of PEA-15 phosphorylation changes upon depolymerization or stabilization of tubulins, indicating that PEA-15 co-localizes with microtubules. The first 80 amino acids of PEA-15 correspond to the death effector domain (DED), which is a domain found in proteins that regulate apoptotic signaling pathways. The DED domain is necessary for PEA-15 to block Ras suppression. Although PEA-15 is predominantly expressed in the central nervous system, low levels of PEA-15 are expressed in liver and kidney, and higher levels in muscle. PEA-15 is also referred to as PED, phosphoprotein enriched in diabetes, for its elevated expression in type 2 diabetic patients.

Long Name

Phosphoprotein Enriched in Astrocytes 15

Alternate Names

HMAT1, HUMMAT1H, MAT1H, PEA15, PED

Gene Symbol

PEA15

Additional PEA-15 Products

Product Documents for PEA-15 Antibody (4D117) - BSA Free

Certificate of Analysis

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Product Specific Notices for PEA-15 Antibody (4D117) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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