PFKP Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-92256

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PFKP Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SILGTKRVLPGKYLEEIATQMRTHSINALLIIGGFEAYLGLLELSAAREKHEEFCVPMVMVPATVSNNVPGS

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (83%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PFKP Antibody - BSA Free

Immunohistochemistry-Paraffin: PFKP Antibody [NBP1-92256]

Immunohistochemistry-Paraffin: PFKP Antibody [NBP1-92256]

Immunohistochemistry-Paraffin: PFKP Antibody [NBP1-92256] - Staining of human hippocampus shows strong cytoplasmic and dot like nucleolar positivity in neuronal cells and strong cytoplasmic staining in glial cells.
PFKP Antibody - BSA Free Immunohistochemistry: PFKP Antibody - BSA Free [NBP1-92256]

Immunohistochemistry: PFKP Antibody - BSA Free [NBP1-92256]

Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
PFKP Antibody - BSA Free Immunohistochemistry: PFKP Antibody - BSA Free [NBP1-92256]

Immunohistochemistry: PFKP Antibody - BSA Free [NBP1-92256]

Staining of human cerebral cortex shows strong cytoplasmic positivity in glial cells.
PFKP Antibody - BSA Free Immunohistochemistry: PFKP Antibody - BSA Free [NBP1-92256]

Immunohistochemistry: PFKP Antibody - BSA Free [NBP1-92256]

Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
PFKP Antibody - BSA Free Immunohistochemistry: PFKP Antibody - BSA Free [NBP1-92256]

Immunohistochemistry: PFKP Antibody - BSA Free [NBP1-92256]

Staining of human tonsil shows strong cytoplasmic positivity in germinal center cells.
PFKP Antibody - BSA Free Western Blot: PFKP Antibody - BSA Free [NBP1-92256]

Western Blot: PFKP Antibody - BSA Free [NBP1-92256]

Analysis in control (vector only transfected HEK293T lysate) and PFKP over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).

Applications for PFKP Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PFKP

The PFKP gene encodes the platelet isoform of phosphofructokinase (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase, EC 2.7.1.11). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The PFKP gene, which maps to chromosome 10p, is also expressed in fibroblasts. See also the muscle (PFKM; MIM 610681) and liver (PFKL; MIM 171860) isoforms of phosphofructokinase, which map to chromosomes 12q13 and 21q22, respectively. Vora (1981) [PubMed 6451249] determined that full tetrameric phophofructokinase enzyme expressed in platelets can be composed of subunits P4, P3L, and P2L2.[supplied by OMIM]

Alternate Names

6-phosphofructokinase type C, 6-phosphofructokinase, platelet type, EC 2.7.1, EC 2.7.1.11, PFK-CFLJ40226, PFKFplatelet type, Phosphofructokinase 1, phosphofructokinase, platelet, phosphohexokinase

Gene Symbol

PFKP

Additional PFKP Products

Product Documents for PFKP Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PFKP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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