PGD Antibody (9A2M7)

Novus Biologicals | Catalog # NBP3-15277

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9A2M7 expressed in HEK293
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Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 384-483 of human PGD (P52209). PELQNLLLDDFFKSAVENCQDSWRRAVSTGVQAGIPMPCFTTALSFYDGYRHEMLPASLIQAQRDYFGAHTYELLAKPGQFIHTNWTGHGGTVSSSSYNA

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PGD Antibody (9A2M7)

Western Blot: PGD Antibody (9A2M7) [NBP3-15277]

Western Blot: PGD Antibody (9A2M7) [NBP3-15277]

Western Blot: PGD Antibody (9A2M7) [NBP3-15277] - Western blot analysis of extracts of various cell lines, using PGD antibody (NBP3-15277) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Immunoprecipitation: PGD Antibody (9A2M7) [NBP3-15277]

Immunoprecipitation: PGD Antibody (9A2M7) [NBP3-15277]

Immunoprecipitation: PGD Antibody (9A2M7) [NBP3-15277] - Immunoprecipitation analysis of 300ug extracts of Jurkat cells using 3ug PGD antibody (NBP3-15277). Western blot was performed from the immunoprecipitate using PGD antibody (NBP3-15277) at a dilition of 1:1000.
PGD Antibody (9A2M7)

Immunocytochemistry/ Immunofluorescence: PGD Antibody (9A2M7) [NBP3-15277] -

Immunocytochemistry/ Immunofluorescence: PGD Antibody (9A2M7) [NBP3-15277] - Immunofluorescence analysis of PC-12 cells using PGD Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
PGD Antibody (9A2M7)

Immunocytochemistry/ Immunofluorescence: PGD Antibody (9A2M7) [NBP3-15277] -

Immunocytochemistry/ Immunofluorescence: PGD Antibody (9A2M7) [NBP3-15277] - Immunofluorescence analysis of NIH/3T3 cells using PGD Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for PGD Antibody (9A2M7)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: 6-Phosphogluconate Dehydrogenase/PGD

6-phosphogluconate dehydrogenase is the second dehydrogenase in the pentose phosphate shunt. Deficiency of this enzyme is generally asymptomatic, and the inheritance of this disorder is autosomal dominant. Hemolysis results from combined deficiency of 6-phosphogluconate dehydrogenase and 6-phosphogluconolactonase suggesting a synergism of the two enzymopathies.

Long Name

6-Phosphogluconate Dehydrogenase

Alternate Names

6PGD, PGDH

Gene Symbol

PGD

Additional 6-Phosphogluconate Dehydrogenase/PGD Products

Product Documents for PGD Antibody (9A2M7)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for PGD Antibody (9A2M7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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