PGK1 Antibody (GT21612) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-13618

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Predicted:

Bovine (96%), Chimpanzee (100%), Mouse (97%), Porcine (96%), Rhesus Macaque (100%), Sheep (96%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # GT21612

Format

Azide and BSA Free
View all PGK1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human PGK1. The exact sequence is proprietary.

Reactivity Notes

Zebrafish (87%), Xenopus laevis (84%), Chicken (88%)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Description

Centrifuge briefly prior to opening.

Scientific Data Images for PGK1 Antibody (GT21612) - Azide and BSA Free

Western Blot: PGK1 Antibody (GT21612) [NBP3-13618]

Western Blot: PGK1 Antibody (GT21612) [NBP3-13618]

Western Blot: PGK1 Antibody (GT21612) [NBP3-13618] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody [GT21612] (NBP3-13618) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: PGK1 Antibody (GT21612) [NBP3-13618]

Immunohistochemistry-Paraffin: PGK1 Antibody (GT21612) [NBP3-13618]

Immunohistochemistry-Paraffin: PGK1 Antibody (GT21612) [NBP3-13618] - PGK1 antibody [GT21612] detects PGK1 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. PGK1 stained by PGK1 antibody [GT21612] (NBP3-13618) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: PGK1 Antibody (GT21612) [NBP3-13618]

Western Blot: PGK1 Antibody (GT21612) [NBP3-13618]

Western Blot: PGK1 Antibody (GT21612) [NBP3-13618] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody [GT21612] (NBP3-13618) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.

Applications for PGK1 Antibody (GT21612) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PGK1

PGK1 is encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. This gene lies on the X-chromosome, while a related pseudogene also has been found on the X-chromosome and another on chromosome 19. [provided by RefSeq]

Long Name

Phosphoglycerate Kinase-1

Alternate Names

MIG10, PGKA, PRP2

Gene Symbol

PGK1

Additional PGK1 Products

Product Documents for PGK1 Antibody (GT21612) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PGK1 Antibody (GT21612) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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