Phalloidin-FITC

Tocris Bioscience | Catalog # 5782

Green-fluorescent cytoskeleton stain. Binds and labels F-actin. Used for: F-actin staining. Application: fluorescent microscopy, immunofluorescence (IHC, ICC)
Tocris Bioscience

Key Product Details

Description

Green-fluorescent cytoskeleton stain. Binds and labels F-actin. Used for: F-actin staining. Application: fluorescent microscopy, immunofluorescence (IHC, ICC)

Wavelength

Excitation = 496 nm, Emission = 516 nm
View all Fluorescent Actin Probes »

Product Description

Key information

: Phalloidin-FITC is a green-fluorescent cytoskeleton stain. It binds and labels specifically F-actin.

Used for: Phalloidin-FITC can be used to visualize and quantify F-actin in tissue sections, cell cultures or cell-free experiments with optimal results in formaldehyde-fixed and permeabilized cells.

Application: fluorescent microscopy, immunofluorescence (IHC, ICC).

Properties and Photophysical Data: Phalloidin-FITC is composed of the F-actin probe, Phalloidin (Cat. No. 4535), conjugated to Fluorescein isothiocyanate (FITC; Cat. No. 5440) dye. Phalloidin binds F-actin with high selectivity allowing Phalloidin-FITC to stain actin with high-contrast discrimination. Excitation and emission maxima (λ) are 496 nm and 516 nm, respectively.

We suggest making up a stock solution of this product by dissolving the vial contents in 1.5 mL methanol or DMSO.

For more information on how to use Phalloidin-FITC, please see our protocol.

Scientific Data Images for Phalloidin-FITC

Applications in HeLa Cells for Phalloidin-FITC

Applications in HeLa Cells for Phalloidin-FITC.

Application of Phalloidin-FITC: HeLa cells were labeled with 150 nM Phalloidin-FITC for 20 minutes. Counterstained with DAPI (Catalog # 5748).

Optical Data

λabs 496 nm
λem 516 nm
Application Fluorescent Microscopy, Immunofluorescence (IHC, ICC).

Spectra Viewer

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Product Specifications for Phalloidin-FITC

Molecular Weight

1177.26

Formula

C56H60N10O15S2

Storage

Store at -20°C

Purity

≥90% (HPLC)

Chemical Name

7-[(4R)-N5-[[(3',6'-Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl)amino]thioxomethyl]-4-hydroxy-4-methyl-L-ornithine]phalloidin

CAS Number

915026-99-2

PubChem ID

131676694

InChI Key

CJEWZWZNRFVJSM-PNHWZQMUSA-N

SMILES

C[C@H](O)[C@H]1NC(=O)C(C)NC(=O)[C@H](C[C@@](C)(O)CNC(=S)NC2=CC(C(O)=O)=C(C=C2)C2=C3C=CC(=O)C=C3OC3=C2C=CC(O)=C3)NC(=O)[C@@H]2CC3=C(NC4=C3C=CC=C4)SC[C@@H](NC1=O)C(=O)N1C[C@H](O)C[C@H]1C(=O)N[C@@H](C)C(=O)N2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 5 publications citing the usage of this product.

There are currently no references for this product.

Product Documents for Phalloidin-FITC

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Phalloidin-FITC

For research use only

Citations for Phalloidin-FITC

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