Phenylalanine Hydroxylase Antibody

Novus Biologicals | Catalog # NBP1-52084

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Predicted:

Bovine (100%), Canine (100%), Mouse (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Phenylalanine Hydroxylase Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-ESRPSRLKKDE corresponding to internal region according to NP_000268.1.

Epitope

C-ESRPSRLKKDE

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Phenylalanine Hydroxylase Antibody

Western Blot: Phenylalanine Hydroxylase Antibody [NBP1-52084]

Western Blot: Phenylalanine Hydroxylase Antibody [NBP1-52084]

Western Blot: Phenylalanine Hydroxylase Antibody [NBP1-52084] - Staining of Rat Kidney lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.
Western Blot: Phenylalanine Hydroxylase Antibody [NBP1-52084]

Western Blot: Phenylalanine Hydroxylase Antibody [NBP1-52084]

Western Blot: Phenylalanine Hydroxylase Antibody [NBP1-52084] - Staining of Human Liver lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.

Applications for Phenylalanine Hydroxylase Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:128000

Western Blot

0.1 - 0.3 ug/mL
Application Notes
Western blot: Approx 48 kDa band observed in Human Liver lysates and approx. 50-52 kDa in Rat Kidney lysates and in preliminary testing of Mouse Liver lysate (calculated MW of 51.9 kDa according to Hyman NP_000268.1 and Rat NP_036751.2).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Phenylalanine Hydroxylase

The PAH gene encodes the enzyme phenylalanine hydroxylase (PAH), which converts phenylalanine to tyrosine and is the rate-limiting enzyme in phenylalanine catabolism. Mammalian PAH is a soluble, homotetrameric protein which is abundantly expressed in human liver. Deficiency of PAH activity results in the autosomal recessive disorder phenylketonuria (PKU), which is characterized by mental retardation unless a low phenylalanine diet is introduced early in life. The PAH gene, which maps to human chromosome 12q23.2, contains all the genetic information necessary to code for functional PAH, demonstrating that a single gene is involved in the classic disease phenotype. Numerous mutations can impair the PAH gene, which result in decreased enzyme activity and give rise to varying degrees of PKU. Multiple isozymes of PAH have been reported to exist, but these are most likely allelic variants of PAH that produce protein subunits with slightly different charge and electrophoretic migration.

Alternate Names

EC 1.14.16, EC 1.14.16.1, PH, phe-4-monooxygenase, phenylalanine 4-monooxygenase, phenylalanine hydroxylase, phenylalanine-4-hydroxylase, PKU, PKU1

Entrez Gene IDs

5053 (Human); 18478 (Mouse); 24616 (Rat)

Gene Symbol

PAH

UniProt

NP_000268.1 (Human)

Additional Phenylalanine Hydroxylase Products

Product Documents for Phenylalanine Hydroxylase Antibody

Certificate of Analysis

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Product Specific Notices for Phenylalanine Hydroxylase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Phenylalanine Hydroxylase Antibody

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