Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all PHF1 Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human PHF1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for PHF1 Antibody - Azide and BSA Free

Western Blot: PHF1 Antibody [NBP2-98955]

Western Blot: PHF1 Antibody [NBP2-98955]

Western Blot: PHF1 Antibody [NBP2-98955] - Anti-PHF1 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lane B: MCF7 Whole Cell Lysate. Lane C: NCI-H1299 Whole Cell Lysate. Lane D: A431 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 62 kDa. Observed band size: 67 kDa (We are unsure as to the identity of these extra bands).
PHF1 Antibody - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: PHF1 Antibody [NBP2-98955] -

Immunocytochemistry/Immunofluorescence: PHF1 Antibody [NBP2-98955] - Immunofluorescence staining of PHF1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98955 antibody (1:5000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to nucleus.

Applications for PHF1 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1500-1:50000

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PHF1

PHF1 encodes a protein with significant sequence similarity to Drosophila Polycomblike. The encoded protein contains a zinc finger-like PHD (plant homeodomain) finger which is distinct from other classes of zinc finger motifs and which shows the typical Cys4-His-Cys3 arrangement. PHD finger genes are thought to belong to a diverse group of transcriptional regulators possibly affecting eukaryotic gene expression by influencing chromatin structure. Two transcript variants have been found for this gene.

Alternate Names

hPCl1, MTF2L2, PCL1, PHD finger protein 1, PHF2, Polycomb-like protein 1, Protein PHF1

Gene Symbol

PHF1

Additional PHF1 Products

Product Documents for PHF1 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PHF1 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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