PHF23 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-28746
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 250 and 300 of human PHD finger protein 23 using the numbering given in entry NP_077273.2 (GeneID 79142).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PHF23 Antibody - BSA Free
Western Blot: PHF23 Antibody [NBP1-28746]
Western Blot: PHF23 Antibody [NBP1-28746] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PHF23 antibody NBP1-28746 used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 10 ug/mg lysate. PHF23 was also immunoprecipitated by rabbit anti-PHF23 antibody NBP1-28747, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).Applications for PHF23 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
10 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: PHF23
Alternate Names
FLJ16355, FLJ22884, hJUNE-1b, MGC2941, PDH-containing protein JUNE-1, PHD finger protein 23, PHD finger protein 23a
Entrez Gene IDs
79142 (Human)
Gene Symbol
PHF23
UniProt
Additional PHF23 Products
Product Documents for PHF23 Antibody - BSA Free
Certificate of Analysis
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Product Specific Notices for PHF23 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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