Phos‑ERK1 (T202/Y204)/ERK2 (T185/Y187) Alexa Fluor® 488‑conjugated Antibody

Catalog # Availability Size / Price Qty
IC7806G
Detection of Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) in Human PBMCs by Flow Cytometry.
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Phos‑ERK1 (T202/Y204)/ERK2 (T185/Y187) Alexa Fluor® 488‑conjugated Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat ERK1 and ERK2 dually phosphorylated at T202/Y204 or T185/Y187, respectively.
Source
Monoclonal Rabbit IgG Clone # 269434
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Phosphopeptide containing the ERK1 T202/Y204 site
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either untreated (light orange filled histogram) or treated with 50 ng/mL PMA and 200 ng/mL Calcium Ionomycin for 10 minutes (dark orange filled histogram) were stained with Rabbit Anti-Human/Mouse/Rat Phospho-ERK1/ERK2 (ERK1 T202/Y204, ERK2 T185/Y187) Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC7806G) or isotype control antibody (Catalog # IC105G, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with ice-cold methanol. View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: ERK1/ERK2

ERK1 and ERK2 (also known as MAPK3 and MAPK1, respectively) are 44- and 42-kDa Ser/Thr kinases, respectively. They are part of the Ras-Raf-ERK signal transduction cascade often found downstream of growth factor receptor activation. ERK1 and ERK2 were initially isolated and cloned as kinases activated in response to Insulin and NGF. They are expressed in most, if not all, mammalian tissues. Dual threonine and tyrosine phosphorylation activate both ERKs, at Thr202/Tyr204 for human ERK1 and Thr185/Tyr187 for human ERK2. The two proteins share 83% amino acid identity, differing mainly at the N- and C-termini.

Long Name
Extracellular Signal-regulated Kinase 1/2
Alternate Names
ERK1/ERK2

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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