Phospho-p53 (S15) PE-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: p53

p53 is a 52-55 kDa member of the p53 family of proteins. It is ubiquitously expressed, being found in both cytoplasm (as a monomer) and nucleus (as an oligomer/tetramer). Human p53 is 393 amino acids in length and contains multiple domains that interact with multiple binding partners. There are numerous posttranslational modification sites, including multiple phosphorylation, acetylation, NEDDylation and ubiquitination sites, plus one SUMOylation and two methylation sites. N-terminal modifications tend to stabilize p53, while C-terminal modifications impact molecule oligomerization, DNA-binding and ubiquitination. Phosphorylation on Ser15 inhibits MAM2 modification of p53, thereby extending its half-life. Notably, there are nine possible p53 isoforms, six of which do not contain Ser15 of the standard (or full-length) form. Thus, they are unlikely to show strong positive staining that is associated with the phosphorylation of Ser15. p53 is best known as a preserver of cell genome integrity. In the nucleus, it stimulates needed repairs to DNA and, if necessary, cease cell division and/or initiate apoptosis. In addition, it has at least one role in the cytoplasm where it is known to regulate glucose consumption.

Product Datasheets

Related Research Areas

• Apoptosis Transcription Factors and Regulators
• Cancer Biomarkers
• Cancer Stem Cell Transcription Factors
• Cell Cycle
• Checkpoint
• DNA Damage and Repair
• Epithelial Stem Cell Transcription Factors
• Hematopoietic Stem Cell Transcription Factors and Regulators
• Immunoglobulin-Like Domain Transcription Factors
• Mesenchymal Stem Cell Transcription Factors
• Neural Stem Cell Transcription Factors
• p53 Pathway
• Parkinson's Disease Related Molecules
• Pluripotent Stem Cell Transcription Factors
• TNF Superfamily Regulators
• Transcription Factors and Regulators
• Transcription Factors in the Akt Pathway