Phospho-p53 (S15) PE‑conjugated Antibody

R&D Systems | Catalog # IC1839P

R&D Systems
Discontinued Product
IC1839P has been discontinued. View all p53 products.

Key Product Details

Species Reactivity

Human

Applications

Intracellular Staining by Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 261352
View all p53 Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing the human p53 S15 site
Accession # P04637

Specificity

Detects human p53 that is phosphorylated at S15 in Western blots. In Western blots, no cross-reactivity with human p53 that is unphosphorylated at S15 is detected.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Phospho-p53 (S15) PE‑conjugated Antibody

Detection of p53 antibody in Camptothecin-treated MCF-7 Human Cell Line antibody by Flow Cytometry.

Detection of p53 in Camptothecin-treated MCF‑7 Human Cell Line by Flow Cytometry.

MCF-7 human breast cancer cell line was treated with Camptothecin overnight (filled histogram) or untreated (open histogram), then stained with Mouse Anti-Human Phospho-p53 (S15) PE-conjugated Monoclonal Antibody (Catalog # IC1839P) or isotype control antibody (Catalog # IC002P, data not shown). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Phospho-p53 (S15) PE‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: MCF‑7 human breast cancer cell line treated with Camptothecin and fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: p53

p53 is a 52-55 kDa member of the p53 family of proteins. It is ubiquitously expressed, being found in both cytoplasm (as a monomer) and nucleus (as an oligomer/tetramer). Human p53 is 393 amino acids in length and contains multiple domains that interact with multiple binding partners. There are numerous posttranslational modification sites, including multiple phosphorylation, acetylation, NEDDylation and ubiquitination sites, plus one SUMOylation and two methylation sites. N-terminal modifications tend to stabilize p53, while C-terminal modifications impact molecule oligomerization, DNA-binding and ubiquitination. Phosphorylation on Ser15 inhibits MAM2 modification of p53, thereby extending its half-life. Notably, there are nine possible p53 isoforms, six of which do not contain Ser15 of the standard (or full-length) form. Thus, they are unlikely to show strong positive staining that is associated with the phosphorylation of Ser15. p53 is best known as a preserver of cell genome integrity. In the nucleus, it stimulates needed repairs to DNA and, if necessary, cease cell division and/or initiate apoptosis. In addition, it has at least one role in the cytoplasm where it is known to regulate glucose consumption.

Alternate Names

BCC7, LFS1, TP53, TRP53

Entrez Gene IDs

7157 (Human); 22059 (Mouse); 24842 (Rat)

Gene Symbol

TP53

UniProt

P04637

Additional p53 Products

Product Documents for Phospho-p53 (S15) PE‑conjugated Antibody

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Product Specific Notices for Phospho-p53 (S15) PE‑conjugated Antibody

For research use only

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