Phospho-p53 (S15) PE-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human p53 that is phosphorylated at S15 in Western blots. In Western blots, no cross-reactivity with human p53 that is unphosphorylated at S15 is detected.
  • Source
    Monoclonal Mouse IgG1 Clone # 261352
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Phosphopeptide containing the human p53 S15 site
    Accession # P04637
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of p53 in Camptothecin-treated MCF‑7 Human Cell Line by Flow Cytometry. MCF‑7 human breast cancer cell line was treated with Camptothecin overnight (filled histogram) or untreated (open histogram), then stained with Mouse Anti-Human Phospho-p53 (S15) PE‑conjugated Monoclonal Antibody (Catalog # IC1839P) or isotype control antibody (Catalog # IC002P, data not shown). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: p53
p53 is a 52-55 kDa member of the p53 family of proteins. It is ubiquitously expressed, being found in both cytoplasm (as a monomer) and nucleus (as an oligomer/tetramer). Human p53 is 393 amino acids in length and contains multiple domains that interact with multiple binding partners. There are numerous posttranslational modification sites, including multiple phosphorylation, acetylation, NEDDylation and ubiquitination sites, plus one SUMOylation and two methylation sites. N-terminal modifications tend to stabilize p53, while C-terminal modifications impact molecule oligomerization, DNA-binding and ubiquitination. Phosphorylation on Ser15 inhibits MAM2 modification of p53, thereby extending its half-life. Notably, there are nine possible p53 isoforms, six of which do not contain Ser15 of the standard (or full-length) form. Thus, they are unlikely to show strong positive staining that is associated with the phosphorylation of Ser15. p53 is best known as a preserver of cell genome integrity. In the nucleus, it stimulates needed repairs to DNA and, if necessary, cease cell division and/or initiate apoptosis. In addition, it has at least one role in the cytoplasm where it is known to regulate glucose consumption.
  • Entrez Gene IDs:
    7157 (Human); 22059 (Mouse); 24842 (Rat)
  • Alternate Names:
    Antigen NY-CO-13; BCC7; FLJ92943; LFS1; LFS1TRP53; p53 tumor suppressor; p53; P53cellular tumor antigen p53; Phosphoprotein p53; TP53; transformation-related protein 53; TRP53; tumor protein p53; Tumor suppressor p53
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 PE-conjugated Antibody

Ctrl IC002P 38  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 5
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009 3
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007 2
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