Phospho-STAT6 (Y641) PE‑conjugated Antibody

Name: Phospho-STAT6 (Y641) PE‑conjugated Antibody
Brand: R&D Systems
SKU: IC37173P
Price: 199 USD
Availability: InStock

R&D Systems | Catalog # IC37173P

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Product Documents

Recombinant Monoclonal Antibody.

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Product Specifications
Scientific Data
Applications
Spectra Viewer
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Intracellular Staining by Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1248D

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Product Specifications

Immunogen

Phosphopeptide containing human STAT6 Y641 site.
Accession # P42226

Specificity

Detects human STAT6 when phosphorylated at Y641.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Phospho-STAT6 (Y641) PE‑conjugated Antibody

Detection of STAT6 in Human peripheral blood mononuclear cells (PBMCs) by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) either (A) Th2-stimulated or (B) unstimulated were stained with Rabbit Anti-Human Phospho-STAT6 (Y641) PE-conjugated Monoclonal Antibody (Catalog # IC37173P) and Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB3791A). Quadrant markers were set based on control antibody staining (Catalog # IC1051P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with methanol. View our protocol for Staining Intracellular Molecules.

Applications for Phospho-STAT6 (Y641) PE‑conjugated Antibody

Application

Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells
Sample: Th2-stimulated human peripheral blood mononuclear cells (PBMCs) fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with methanol

Spectra Viewer

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Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: STAT6

Signal Transducer and Activator of Transcription 6 (STAT6) mediates the signaling of cytokines such as IL-4 and IL-13. STAT6 acts as a signal transducer in the cytoplasm and, upon phosphorylation at Y641, translocates to the nucleus and binds to the DNA consensus site TTCN₄GAA. Knockout studies in mice suggest that STAT6 functions in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins.

Long Name

Signal Transducer and Activator of Transcription 6

Alternate Names

D12S1644, EC 2.4.1.227, EC 2.7.7.6, IL-4 Stat, IL-4-STAT, signal transducer and activator of transcription 6, signal transducer and activator of transcription 6, interleukin-4 induced, STAT, interleukin-4 induced, STAT, interleukin4-induced, STAT6B, STAT6C, transcription factor IL-4 STAT