Phospholamban [p Ser16] Antibody (HL1710) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25600

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse

Predicted:

Bovine (100%), Canine (100%), Human (100%), Porcine (100%), Rabbit (100%), Rat (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1710 expressed in HEK293

Format

Azide and BSA Free
View all Phospholamban Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser16 of human Phospholamban. The exact sequence is proprietary

Modification

p Ser16

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Phospholamban [p Ser16] Antibody (HL1710) - Azide and BSA Free

Phospholamban [p Ser16] Antibody (HL1710) - Azide and BSA Free

Western Blot: Phospholamban [p Ser16] Antibody (HL1710) - Azide and BSA Free [NBP3-25600] -

Western Blot: Phospholamban [p Ser16] Antibody (HL1710) - Azide and BSA Free [NBP3-25600] - Untreated (-) and treated (+) Mouse tissue extract (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Phospholamban (phospho Ser16) antibody [HL1710] (NBP3-25600) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Phospholamban [p Ser16] Antibody (HL1710) - Azide and BSA Free

Application
Recommended Usage

Western Blot

Assay dependent

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Phospholamban

Phospholamban (PLB) is a 52 amino acid phosphoprotein which regulates the calcium pump of cardiac sarcoplasmic reticulum (SR). PLB is an oligomer of five identical subunits each having a cytoplasmic and transmembrane domain. The cytoplasmic domain (residues 1-25) contains the phosphorylation sites and is highly basic and readily cleaved by proteases; whereas the transmembrane domain (residues 25-52) is mostly hydrophobic, protease resistant and stabilizes the pentamer. Following adrenergic stimulation of cardiac muscle, PLB is phosphorylated at Ser16 and at Thr17 which is correlated with stimulation of calcium transport activity across the SR membrane and relaxation of cardiac fibers.

Alternate Names

CMD1PPLBcardiac phospholamban, phospholamban

Gene Symbol

PLN

Additional Phospholamban Products

Product Documents for Phospholamban [p Ser16] Antibody (HL1710) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Phospholamban [p Ser16] Antibody (HL1710) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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