Phospholipase A2 XII Antibody (7C7C9) - BSA Free

Novus Biologicals | Catalog # NBP2-37258

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 7C7C9

Format

BSA Free
View all Phospholipase A2 XII Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Phospholipase A2 XII (AA: 21-189) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Phospholipase A2 XII Antibody (7C7C9) - BSA Free

Western Blot: Phospholipase A2 XII Antibody (7C7C9)BSA Free [NBP2-37258]

Western Blot: Phospholipase A2 XII Antibody (7C7C9)BSA Free [NBP2-37258]

Western Blot: Phospholipase A2 XII Antibody (7C7C9) [NBP2-37258] - Western blot analysis using PLA2G12A mAb against HEK293 (1) and PLA2G12A (AA: 21-189)-hIgGFc transfected HEK293 (2) cell lysate.
Flow Cytometry: Phospholipase A2 XII Antibody (7C7C9) - BSA Free [NBP2-37258]

Flow Cytometry: Phospholipase A2 XII Antibody (7C7C9) - BSA Free [NBP2-37258]

Flow Cytometry: Phospholipase A2 XII Antibody (7C7C9) [NBP2-37258] - Flow cytometric analysis of Hela cells using PLA2G12A mouse mAb (green) and negative control (red).
Western Blot: Phospholipase A2 XII Antibody (7C7C9)BSA Free [NBP2-37258]

Western Blot: Phospholipase A2 XII Antibody (7C7C9)BSA Free [NBP2-37258]

Western Blot: Phospholipase A2 XII Antibody (7C7C9) [NBP2-37258] - Western blot analysis using PLA2G12A mAb against human PLA2G12A (AA: 21-189) recombinant protein. (Expected MW is 44.5 kDa)
ELISA: Phospholipase A2 XII Antibody (7C7C9) - BSA Free [NBP2-37258]

ELISA: Phospholipase A2 XII Antibody (7C7C9) - BSA Free [NBP2-37258]

ELISA: Phospholipase A2 XII Antibody (7C7C9) [NBP2-37258] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for Phospholipase A2 XII Antibody (7C7C9) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Phospholipase A2 XII

Secreted phospholipase A2 (sPLA2) enzymes liberate arachidonic acid from phospholipids for production of eicosanoids and exert a variety of physiologic and pathologic effects. Group XII sPLA2s, such as PLA2G12A, have relatively low specific activity and are structurally and functionally distinct from other sPLA2s (Gelb et al., 2000 [PubMed 11031251]).[supplied by OMIM]

Alternate Names

EC 3.1.1.4, group XII secreted phospholipase A2, group XIIA secreted phospholipase A2, group XIIA secretory phospholipase A2, GXII, GXII sPLA2, Phosphatidylcholine 2-acylhydrolase 12A, phospholipase A2, group XII, phospholipase A2, group XIIA, PLA2G12, ROSSY, sPLA2-XII

Gene Symbol

PLA2G12A

Additional Phospholipase A2 XII Products

Product Documents for Phospholipase A2 XII Antibody (7C7C9) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Phospholipase A2 XII Antibody (7C7C9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Phospholipase A2 XII Antibody (7C7C9) - BSA Free

Showing  1 - 2 of 2 FAQs Showing All

  • Q:

    Our customer asked about the cross reactivity of PLA2G12A antibodies. Do you have any info if these antibodies recognize other phospholipases?

    A: Unless specifically indicated on the datasheet they are not tested to recognize other phospholipases. The immunogen information is present for all of those so that might give some clues to the customer as to whether they might have some reactivity, but as far as direct testing is concerned on the other phospholipases this has not been performed by the lab.

  • Q: Our customer asked about the cross reactivity of PLA2G12A antibodies. Do you have any info if these antibodies?

    A: Unless specifically indicated on the datasheet they are not tested to recognize other phospholipases. The immunogen information is present for all of those so that might give some clues to the customer as to whether they might have some reactivity, but as far as direct testing is concerned on the other phospholipases this has not been performed by the lab.

  • Q:

    Our customer asked about the cross reactivity of PLA2G12A antibodies. Do you have any info if these antibodies recognize other phospholipases?

    A: Unless specifically indicated on the datasheet they are not tested to recognize other phospholipases. The immunogen information is present for all of those so that might give some clues to the customer as to whether they might have some reactivity, but as far as direct testing is concerned on the other phospholipases this has not been performed by the lab.

  • Q: Our customer asked about the cross reactivity of PLA2G12A antibodies. Do you have any info if these antibodies?

    A: Unless specifically indicated on the datasheet they are not tested to recognize other phospholipases. The immunogen information is present for all of those so that might give some clues to the customer as to whether they might have some reactivity, but as far as direct testing is concerned on the other phospholipases this has not been performed by the lab.

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