Phosphorylase B Antibody (2E9) - Azide and BSA Free

Novus Biologicals | Catalog # H00005257-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2E9

Format

Azide and BSA Free
View all Phosphorylase B Primary Antibodies »

Product Specifications

Immunogen

PHKB (NP_000284, 984 a.a. ~ 1093 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EDTLGNIDQPQYRQIVVELLMVVSIVLERNPELEFQDKVDLDRLVKEAFNEFQKDQSRLKEIEKQDDMTSFYNTPPLGKRGTCSYLTKAVMNLLLEGEVKPNNDDPCLIS

Specificity

PHKB (2E9)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Phosphorylase B Antibody (2E9) - Azide and BSA Free

Western Blot: Phosphorylase B Antibody (2E9) [H00005257-M02]

Western Blot: Phosphorylase B Antibody (2E9) [H00005257-M02]

Western Blot: Phosphorylase B Antibody (2E9.) [H00005257-M02] - Detection against Immunogen (37.84 KDa).

Applications for Phosphorylase B Antibody (2E9) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Phosphorylase B

Phosphorylase B, also known by its gene name, PHKB, is an enzyme localized to the plasma membrane that catalyzes the phosphorylation of the amino acid residue Serine. Phosphorylase B has a 1,093 amino acid long isoform that is 125 kDa (the canonical sequence) and three other isoforms derived from alternative splicing. Current research on PHKB is being performed relating to several diseases and disorders including hepatitis and metabolic disorders, such as hypoglycemia and glycogen storage disease. Phosphorylase B has also been shown to have interactions with B Raf, hnRNP C1 + C2, PASK, and UBE3A in pathways such as Glucose and Glycogen Metabolism, PKA Signaling, cAMP Pathway, Insulin Signaling Pathway and Calcium Signaling Pathway.

Alternate Names

DKFZp781E15103, FLJ41698, phosphorylase b kinase regulatory subunit beta, phosphorylase kinase beta-subunit, Phosphorylase kinase subunit beta, phosphorylase kinase, beta

Entrez Gene IDs

5257 (Human)

Gene Symbol

PHKB

OMIM

172490 (Human)

UniProt

NP_000284 (Human)

Additional Phosphorylase B Products

Product Documents for Phosphorylase B Antibody (2E9) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Phosphorylase B Antibody (2E9) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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