PIASy Antibody

Novus Biologicals | Catalog # NB600-1318

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Parasite - Trypanosoma brucei

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all PIASy/PIAS4 Primary Antibodies »

Product Specifications

Immunogen

This polyclonal antibody was developed against a mixture of synthetic peptides corresponding to amino acids 46-60 (KKIKELYETRYAKKNC) and 187-198 (YSDTSCPQEDQY) of human PIASg; GenBank no. AAD45155.1|AF164437_1. These sequences are 95% and 100% homologous in mouse and rat.

Localization

Nuclear

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PIASy Antibody

Western Blot: PIASy Antibody [NB600-1318]

Western Blot: PIASy Antibody [NB600-1318]

Western Blot: PIASy Antibody [NB600-1318] - Western blot analysis of PIASg in Jurkat cell lysate using NB600-1318 at 2 ug/ml.
Western Blot: PIASy Antibody [NB600-1318]

Western Blot: PIASy Antibody [NB600-1318]

Western Blot: PIASy Antibody [NB600-1318] - Analysis of PIASin A) human liver, B) mouse liver, C) rat liver and D) human Jurkat lysate using this antibody at 5 ug/ml.

Applications for PIASy Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:2000. Use reported in scientific literature (Ihara et al (2005))

Western Blot

3 - 5 ug/ml
Application Notes
Western Blot: Detects a band of approximately 55 kDa.

Reviewed Applications

Read 1 review rated 3 using NB600-1318 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS containing 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PIASy/PIAS4

The interaction of a cytokine with its specific cell surface receptor triggers the activation of intracellular signaling pathways that ultimately program the cellular response. The signal transducers and activators of transcription (STAT) proteins play important roles in the activation of these pathways. Recently, a family of proteins has been identified that inhibits activated STATS and are named as Protein Inhibitors of Activated STAT (PIAS). PIAS proteins comprise a family of five mammalian proteins, which have been identified as potentially important down regulators of the STAT-signaling pathway. The cDNA for mouse and human PIASg encodes a protein of 507 amino acids that is highly homologous to the human protein and is expressed in the mouse as early as d 7.5 of gestation. In situ hybridizations of staged mouse embryos localized the transcript for the PIASg gene to the limbs, neuroepithelium, and the inner root sheath of the hair follicle, suggesting a role in the development of these structures. This protein is also present in hair follicle of human scalp hair and in monkey neuronal cells. Thus PIASg exhibits a highly selective pattern of expression, suggesting that it modulates the response of cells to developmental cues.

Long Name

Protein Inhibitor of Activated STAT, 4

Alternate Names

PIAS4, PIASG, ZMIZ6

Entrez Gene IDs

51588 (Human)

Gene Symbol

PIAS4

UniProt

Q8N2W9 (Human)

Additional PIASy/PIAS4 Products

Product Documents for PIASy Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for PIASy Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for PIASy Antibody (1)

3 out of 5
1 Customer Rating
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2 Stars
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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Jurkat cells
    Species: Human
    Verified Customer | Posted 10/02/2014
    Western for PIASy
    PIASy Antibody NB600-1318

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Protocols

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FAQs

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