PIK3R4 Antibody (5J3U5)

Novus Biologicals | Catalog # NBP3-16612

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5J3U5 expressed in HEK293
View all PIK3R4 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1259-1358 of human PIK3R4 (Q99570). AGSDMKIRFWDLAYPERSYVVAGSTSSPSVSYYRKIIEGTEVVQEIQNKQKVGPSDDTPRRGPESLPVGHHDIITDVATFQTTQGFIVTASRDGIVKVWK

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PIK3R4 Antibody (5J3U5)

Western Blot: PIK3R4 Antibody (5J3U5) [NBP3-16612]

Western Blot: PIK3R4 Antibody (5J3U5) [NBP3-16612]

Western Blot: PIK3R4 Antibody (5J3U5) [NBP3-16612] - Western blot analysis of extracts of various cell lines, using PIK3R4 Rabbit mAb (NBP3-16612) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Immunocytochemistry/ Immunofluorescence: PIK3R4 Antibody (5J3U5) [NBP3-16612]

Immunocytochemistry/ Immunofluorescence: PIK3R4 Antibody (5J3U5) [NBP3-16612]

Immunocytochemistry/Immunofluorescence: PIK3R4 Antibody (5J3U5) [NBP3-16612] - Immunofluorescence analysis of NIH-3T3 cells using PIK3R4 Rabbit mAb (NBP3-16612) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blot: PIK3R4 Antibody (5J3U5) [NBP3-16612]

Western Blot: PIK3R4 Antibody (5J3U5) [NBP3-16612]

Western Blot: PIK3R4 Antibody (5J3U5) [NBP3-16612] - Western blot analysis of extracts of Rat brain, using PIK3R4 Rabbit mAb (NBP3-16612) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Immunocytochemistry/ Immunofluorescence: PIK3R4 Antibody (5J3U5) [NBP3-16612]

Immunocytochemistry/ Immunofluorescence: PIK3R4 Antibody (5J3U5) [NBP3-16612]

Immunocytochemistry/Immunofluorescence: PIK3R4 Antibody (5J3U5) [NBP3-16612] - Immunofluorescence analysis of HeLa cells using PIK3R4 Rabbit mAb (NBP3-16612) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
PIK3R4 Antibody (5J3U5)

Immunocytochemistry/ Immunofluorescence: PIK3R4 Antibody (5J3U5) [NBP3-16612] -

Immunocytochemistry/ Immunofluorescence: PIK3R4 Antibody (5J3U5) [NBP3-16612] - Confocal imaging of NIH/3T3 cells using PIK3R4 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Applications for PIK3R4 Antibody (5J3U5)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PIK3R4

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.

Long Name

Phosphoinositide-3-kinase, Regulatory Subunit 4

Alternate Names

p150, VPS15

Gene Symbol

PIK3R4

Additional PIK3R4 Products

Product Documents for PIK3R4 Antibody (5J3U5)

Certificate of Analysis

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Product Specific Notices for PIK3R4 Antibody (5J3U5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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Associated Pathways

Akt Signaling Pathway Akt Signaling Pathway Thumbnail