PKA C alpha Antibody (6D2.1) - BSA Free

Novus Biologicals | Catalog # NBP2-62195

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 6D2.1

Format

BSA Free
View all PKA C alpha Primary Antibodies »

Product Specifications

Immunogen

Peptide corresponding to amino acids ESPAQNTAHLDQFERIK of human proteinkinase A c alpha (PKAc alpha).

Specificity

The antibody C-50 reacts with Cytokeratin peptides 5 and 18 (58 and 45 kDa). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PKA C alpha Antibody (6D2.1) - BSA Free

Dot Blot: PKA C alpha Antibody (6D2.1) [NBP2-62195] - analysis of GST and GST-fusion proteins using anti-PKAc (6D2.1) and anti-GST (S-tag-05; cat. no. NBP1-48359).The total amount of material spotted on the nitrocellulose membrane is 5 ng/spot.Lane 1: GST-Akt1Lane 2: GST-Akt2Lane 3: GST-Akt3Lane 4: GST-PKAc alphaLane 5: GST-PKAc betaLane 6: GST-PKAc gammaLane 7: GST-MEK 1Lane 8: GST

Applications for PKA C alpha Antibody (6D2.1) - BSA Free

Application
Recommended Usage

Western Blot

1 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PKA C alpha

PKA (Protein Kinase A), also known as cAMP-dependent protein kinase, phosphorylates serine or threonine residues in target protein in response to elevated levels of cAMP. Inactive PKA exists as a tetrameric protein composed of two regulatory (R) subunits and two catalytic (C) subunits. Activation occurs when two cAMP molecules bind to each R subunit which causes a conformational change that releases the active C subunits.

Long Name

cAMP-dependent Protein Kinase Catalytic Subunit alpha

Alternate Names

Cs, Pkaca, PKCA1, PKCD, PRKACA

Gene Symbol

PRKACA

Additional PKA C alpha Products

Product Documents for PKA C alpha Antibody (6D2.1) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for PKA C alpha Antibody (6D2.1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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